Advertisement

Roux's archives of developmental biology

, Volume 199, Issue 4, pp 219–227 | Cite as

Control of the developmental timer forDrosophila pupariation

  • Clifton A. Poodry
  • Daniel F. Woods
Article

Summary

In the insectDrosophila, formation of the puparium marks the onset of metamorphosis and serves as a useful marker for developmental progress. The cells of the adult remain diploid and divide during the larval stage while the larval cells become polytene and do not divide. We use a high dose of gamma-irradiation (10 krad) to selectively delete the imaginal lineage from the developing larvae ofDrosophila melanogaster. We find that animals depleted of imaginal cells including those of the imaginal brain pupariate only if the larval cells are allowed to mature, demonstrating that the larval cells harbor the primary developmental timer for this process. However, proliferating imaginal cells can exert a negative influence on the timing of pupariation.

Key words

Puparium Metamorphosis Irradiation Larva Developmental timer 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arena V (1971) Ionizing radiation and life: An introduction to radiation biology and biological radiotracer methods. Mosby, St. LouisGoogle Scholar
  2. Bodenstein D (1950) The postembryonic development ofDrosophila. In: Demerec M (ed) Biology ofDrosophila. Wiley, New York, pp 275–367Google Scholar
  3. Borst DW, Bollenbacher WE, O'Connor JD, King DS, Fristrom JW (1974) Ecdysone levels during metamorphosis ofDrosophila melanogaster. Dev Biol 39:65–76Google Scholar
  4. Bownes M (1975) A photographic study of development in the living embryo ofDrosophila melanogaster. J Embryol Exp Morphol 32:789–801Google Scholar
  5. Brett WJ (1955) Persistant diurnal rhythmicity inDrosophila emergence. Ann Ent Soc Am 48:119–131Google Scholar
  6. Bryant PJ (1987) Experimental and genetic analysis of growth and cell proliferation inDrosophila imaginal discs. In: Loomis WF (ed) Genetic regulation of development. 45th Ann Symp Soc Dev Biol, AR Liss, Inc, New York, pp 339–372Google Scholar
  7. Bryant PJ, Levinson PY (1985) Intrinsic growth control in the imaginal primordia ofDrosophila, and the autonomous action of a lethal mutation causing overgrowth. Dev Biol 107:355–363PubMedGoogle Scholar
  8. Bryant PJ, Schubiger G (1971) Giant and duplicated imaginal discs in a new lethal mutant ofDrosophila melanogaster. Dev Biol 24:233–263PubMedGoogle Scholar
  9. Bryant PJ, Simpson P (1984) Intrinsic and extrinsic control of growth in developing organs. Quart Rev Biol 59:387–415PubMedGoogle Scholar
  10. Campos-Ortega JC, Hartenstein V (1985) The embryonic development ofDrosophila melanogaster. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  11. Dewes E (1973) Regeneration in transplanted halves of male genital disks and its influences upon duration of development inEphestia kuhniella Z. Roux's Arch Dev Biol 172:349–354Google Scholar
  12. Gateff E (1978) Malignant neoplasms of genetic origin inDrosophila melanogaster. Science 200:1448–1459PubMedGoogle Scholar
  13. Grossfield J (1978) Non-sexual behavior ofDrosophila. In: Ashburner M, Wright TRF (eds) The genetics and biology ofDrosophila, vol 2b. Academic Press, London, pp 3–126Google Scholar
  14. Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92Google Scholar
  15. Haynie JL, Bryant PJ (1977) The effects of X-rays on the proliferation dynamics of cells in the imaginal wing disc ofDrosophila melanogaster. Roux's Arch Dev Biol 183:85–100Google Scholar
  16. Heinrich VC, Pak MD, Gilbert LI (1987) Neural factors that stimulate ecdysteroid synthesis by the larval ring gland ofDrosophila melanogaster. J Comp Physiol B 157:543–549PubMedGoogle Scholar
  17. James AA, Bryant PJ (1981) A quantitative study of cell death and mitotic inhibition in -irradiated imaginal wing discs ofDrosophila melanogaster. Radiation Res 87:552–564PubMedGoogle Scholar
  18. Madhavan MM, Schneiderman HA (1977) Histological analysis of the dynamics of growth of imaginal discs and histoblast nests during the larval development ofDrosophila melanogaster. Roux's Arch Dev Biol 183:269–305Google Scholar
  19. Nieuwkoop PD, Faber J (1967) Normal table ofXenopus laevis. 2nd ed. North-Holland, AmsterdamGoogle Scholar
  20. Poodry CA (1975) Autonomous and non-autonomous cell death in the metamorphosis of the epidermis ofDrosophila. Roux's Arch Dev Biol 178:333–336Google Scholar
  21. Poodry CA (1980) Epidermis: Morphology and development. In: Ashburner M, Wright TRF (eds) Genetics and biology ofDrosophila, vol 2. Academic Press, New York, pp 407–441Google Scholar
  22. Rahn P (1972) Untersuchungen zur Entwicklung von Ganz- and Teilimplantaten der Flügelimaginalscheibe vonEphestia kühniella Z. Roux's Arch Dev Biol 170:48–72Google Scholar
  23. Saunders DS (1982) Insect clocks. 2nd ed. Pergamon Press, OxfordGoogle Scholar
  24. Shearn A, Rice T, Garen A, Gehring W (1971) Imaginal disc abnormalities in lethal mutants ofDrosophila. Proc Natl Acad Sci USA 68:2594–2598PubMedGoogle Scholar
  25. Simpson P, Berreur P, Berreur-Bonnenfant (1980) The initiation of pupariation in Drosophila: Dependence on growth of the imaginal discs. J Embryol Exp Morphol 57:155–165PubMedGoogle Scholar
  26. Steel CGH, Davey KG (1985) Integration in the insect endocrine system. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry, and pharmacology. Pergamon Press, New York, pp 1–35Google Scholar
  27. Stewart M, Murphy C, Fristom JW (1972) The recovery and preliminary characterization of X-chromosome mutants affecting imaginal discs ofDrosophila melanogaster. Dev Biol 27:71–83PubMedGoogle Scholar
  28. Szabad J, Bryant PJ (1982) The mode of action of “discless” mutations in Drosophila melanogaster. Dev Biol 93:240–256PubMedGoogle Scholar
  29. Truman JW, Bate M (1988) Spatial and temporal patterns of neurogenesis in the central nervous system ofDrosophila melanogaster. Dev Biol 125:145–157PubMedGoogle Scholar
  30. Wieschaus E, Nüsslein-Volhard (1986) Looking at Embryos In: Roberts DB (ed) Drosophila: a practical approach. IRL Press, Oxford, pp 197–227Google Scholar
  31. Wigglesworth VB (1972) The principles of insect physiology. 7th ed Chapman and Hall, LondonGoogle Scholar
  32. Woods DF, Bryant PJ (1989) Molecular cloning of the lethal(1)-discs large-1 oncogene ofDrosophila. Dev Biol 134:222–235PubMedGoogle Scholar
  33. Zdarek J (1985) Regulation of pupariation in flies. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry, and pharmacology. Pergamon Press, New York, pp 301–333Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Clifton A. Poodry
    • 1
  • Daniel F. Woods
    • 2
  1. 1.Department of BiologyThimann Laboratories, University of CaliforniaSanta CruzUSA
  2. 2.Developmental Biology CenterUniversity of CaliforniaIrvineUSA

Personalised recommendations