The Histochemical Journal

, Volume 19, Issue 12, pp 671–675 | Cite as

The impact of different fixation procedures on staining of macromolecules in a microtiter system

  • M. R. Barer
  • H. O. Lyon


The effects of formaldehyde, glutaraldehyde, methanol, Clarke's fixative and microwave irradiation on the quantitative staining of proteins (Naphthol Yellow S) and nucleic acids (Ethyl Green—Pyronin) in a cell culture system have been investigated. Overall, glutaraldehyde rapidly yielded the highest and most consistent levels of staining when compared to all other chemical fixatives. Although microwave irradiation was found to be uneven, 4 min exposure to 700W was found to give higher levels of protein staining than those achieveable with glutaraldehyde. Time-dependent processes were observed with all procedures. In addition, dissociations in the trends of protein and nucleic acid staining were observed. It is suggested that these results domonstrate fixation events that have not previously been resolved from the effects of reagent penetration into tissue blocks.


Nucleic Acid Fixation Event Macromolecule Glutaraldehyde Culture System 
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Copyright information

© Chapman and Hall Ltd. 1987

Authors and Affiliations

  • M. R. Barer
    • 1
  • H. O. Lyon
    • 2
  1. 1.Department of Clinical Tropical MedicineThe London School of Hygiene and Tropical MedicineLondonUK
  2. 2.Department of Pathology, Hvidovre HospitalUniversity of CopenhagenHvidovreDenmark

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