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Immunohistochemical localization of plasma retinolbinding protein and prealbumin in human pancreatic islets

Summary

This study was undertaken, employing the immunoenzyme method, to confirm the presence of retinol-binding protein in human pancreatic islets, and to compare its distribution with that of prealbumin, insulin, glucagon, somatostatin and pancreatic polypeptide. It was found that most islet cells contained retinol-binding protein, although centrally located cells showed stronger reactivity than those in the peripheral region. The distribution of each of the five polypeptides differed from that of retinolbinding protein, indicating that these peptides did not cross-react with anti-retinol-binding protein antibody. Islet cells which contained prealbumin, on the other hand, were mostly classified as A cells. Further studies are necessary to confirm whether the islet cells produce retinol-binding protein or only store it.

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References

  1. BUSSOLATI, G., PAPOTTI, M. & SAPINO, A. (1984) Binding of antibodies against human prealbumin to intestinal and bronchial carcinoids and to pancreatic endocrine tumours.Virchows Arch. B. Cell Path. 45, 15–22.

  2. ERLANDSON, S. L. (1980) Types of pancreatic islet cells and their immunocytochemical identification. InThe Pancreas (edited by FITZGERALD, P. J. and MORRISON, A. B.), pp. 140–55. Baltimore and London: Williams and Wilkins.

  3. JACOBSSON, B., PETTERSSON, T., SANDSTEDT, B. & CARLSTRÖEM, A. (1979) Prealbumin in the islets of Langerhans.IRCS Med. Sci-Biochem. 7, 590.

  4. JÖERNVALL, H., CARLSTRÖEM, A., PETTERSSON, T., JACOBSSON, B., PERSSON, M. & MUTT, V. (1981) Structural homologies between prealbumin, gastrointestinal prohormones and other proteins.Nature Lond. 291, 261–3.

  5. KANAI, M., RAZ, A. & GOODMAN, D. S. (1968) Retinolbinding protein: the transport protein for vitamin A in human plasma.J. clin. Invest. 47, 2025–44.

  6. KANAI, M., IWANAGA, T., HAGINO, N. & MUTO, Y. (1978) Retinol-binding protein in tubular proteinuria of patients with Itai-Itai disease.Wld Rev. Nut. Diet. 31, 31–6.

  7. KATOH, M., KANAI, M., KAMEKO, M., OHNO, S., FUJI, Y. & NAGATA, T. (1981) Localization of retinol-binding protein and prealbumin in the human kidney with an unlabeled enzyme immunohistochemical method.Acta histochem. cytochem. 15, 68–75.

  8. KATO, M., KATO, K., BLANER, W. S., CHERTOW, B. S. & GOODMAN, D. S. (1985) Plasma and cellular retinoidbinding proteins and transthyretin (prealbumin) are all localized in the islets of Langerhans in the rat.Proc. natn. Acad. Sci. USA 82, 2488–92.

  9. KATO, M., KATO, K. & GOODMAN, D. S. (1984) Immunocytochemical studies on the localization of plasma and of cellular retinol-binding proteins and transthyretin (prealbumin) in rat liver and kidney.J. Cell Biol. 98, 1696–704.

  10. LIDDLE, C. N., REID, W. A., HORNE, C. H. W. & KENNEDY, J. S. (1983) Prealbumin in pancreatic islet cells.J. Path. 139, 471–2.

  11. MUTO, Y., SMITH, F. R. & GOODMAN, D. S. (1973) Comparative studies of retinol transport in the plasma.J. Lipid Res. 14, 525–32.

  12. NEWCOMER, M. E., LILJAS, A., SUNDELIN, J., RASK, L. & PETERSON, P. A. (1984) Crystallization of and preliminary X-ray data for the plasma retinol-binding protein.J. biol. Chem. 259, 5230–1.

  13. PETERSON, P. A. (1971) Characteristics of vitamin A-transporting protein complex occurring in human serum.J. biol. Chem. 246, 34–43.

  14. POOLE, A. R., DINGLE, J. T., MALLIA, A. K. & GOODMAN, D. S. (1975) The localization of retinol-binding protein in rat liver by immunofluorescence microscopy.J. Cell Sci. 19, 379–94.

  15. RAZ, A., SHIRATORI, T. & GOODMAN, D. S. (1970) Studies on the protein-protein and protein-ligand interactions involved in retinol transport in plasma.J. biol. Chem. 245, 1903–12.

  16. SMITH, F. R. & GOODMAN, D. S. (1971) The effects of diseases of the liver, thyroid, and kidneys on the transport of vitamin A in human plasma.J. clin. Invest. 50, 2426–36.

  17. STERNBERGER, L. A., HARDY, P. H., CUCULIS, J. J. & MEYER, H. G. (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase—antihorseradish peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem. 18, 315–33.

  18. USUDA, N., KAMEKO, M., KANAI, M. & NAGATA, T. (1983) Immunocytochemical demonstration of retinol-binding protein in the lysosome of the proximal tubules of the human kidney.Histochemistry 78, 487–90.

  19. VAHLQUIST, A., PETERSON, P. A. & WIBELL, L. (1973) Metabolism of the vitamin A transporting protein complex. I. Turnover studies in normal persons and in patients with chronic renal failure.Eur. J. clin. Invest. 3, 352–62.

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Kameko, M., Ichikawa, M., Katsuyama, T. et al. Immunohistochemical localization of plasma retinolbinding protein and prealbumin in human pancreatic islets. Histochem J 18, 164–168 (1986). https://doi.org/10.1007/BF01676116

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Keywords

  • Peptide
  • Polypeptide
  • Glucagon
  • Islet Cell
  • Pancreatic Islet