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Sequence analysis of the rDNA spacer ofParacentrotus lividus and observations about pre-rRNA processing

NTS sequence of Paracentrotus lividusrDNA

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Abstract

We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchinParacentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor which has the same 5′ terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.

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Abbreviations

bp:

base pair(s)

Kb:

Kilobase(s) or 1000 bp

nt:

nucleotide(s)

rDNA:

DNA encoding rRNA

rRNA:

ribosomal RNA

S:

sedimentation constant

References

  1. 1.

    Passananti C, Felsani A, Giordano R, Metafora S & Spatafora C (1983) Europ. J. Biochem. 137: 233–239

  2. 2.

    Barbieri R, Duro G, Cantone M, Izzo V, Venturella S & Giudice G (1990) Cell Biol. Internat. Rep. 14: 917–926

  3. 3.

    Taira T, Kato A & Tanifuji S (1988) Mol. Gen. Genet. 213: 170–174

  4. 4.

    Sollner-Webb B & Tower J (1986) Ann. Rev. Biochem. 55: 801–830

  5. 5.

    Haltiner MM, Smale ST & Tjian R (1986) Mol. Cell. Biol. 6: 227–234

  6. 6.

    Windle J & Sollner-Webb B (1986) Mol. Cell. Biol. 6: 1228–1134

  7. 7.

    Giudice G, Pirrone AM, Roccheri M & Trapani M (1973) Biochem. Bioph. Acta 319: 72–80

  8. 8.

    Giudice G (1986) The sea urchin Embryo. A developmental biological system (pp 102–104) Springer-Verlag

  9. 9.

    Sambrook J, Fritsch EF & Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

  10. 10.

    Sanger F, Nicklen S & Coulson AR (1977) Proc. Natl. Acad. Sci. USA 74: 5463–5468

  11. 11.

    Chomezynki P & Sacchi N (1987) Analyt. Biochem. 162: 156–159

  12. 12.

    Feinberg AP & Vogelstein B (1983) Analyt. Biochem. 132: 6–13

  13. 13.

    Duro G, Izzo V, Barbieri R, Cantone M, Costa MA & Giudice G (1991) Analyt. Biochem. 195: 111–115

  14. 14.

    Berk AJ & Sharp PA (1977) Cell 12: 721–727

  15. 15.

    Kuo-Chien H & Stafford DW (1985) Gene 39: 49–54

  16. 16.

    Lanhart P & Reeder RH (1986) Cell 45: 431–443

  17. 17.

    Gurney T Jr (1985) Nucleic Ac. Res. 13: 4905–4919

  18. 18.

    Miller KG & Sollner-Webb B (1981) Cell 27: 165–174

  19. 19.

    Veinot-Drebot LM, Singer RA & Johnston GC (1988) J. Mol. Biol. 199: 107–113

  20. 20.

    Angerer ML, Yang Q, Liesveld J, Kingsley PD & Angerer RC (1992) Devel. Biol. 144: 27–40

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Correspondence to G. Giudice.

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Cantone, M., Barbieri, R., Duro, G. et al. Sequence analysis of the rDNA spacer ofParacentrotus lividus and observations about pre-rRNA processing. Mol Biol Rep 18, 177–182 (1993). https://doi.org/10.1007/BF01674428

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Key words

  • external transcribed spacer
  • non-transcribed spacer
  • Northern-blot hybridization
  • ribosomal RNA
  • sea urchin
  • S1 mapping