Cancer Immunology, Immunotherapy

, Volume 30, Issue 3, pp 185–189 | Cite as

HLA-DR antigen expression on melanoma metastases and the course of the disease

  • Marta Munzarová
  • Daniela Zemanová
  • Aleš Rejthar
  • Zdenek Mechl
  • Věra Kolcová
Original articles


Melanoma cells freshly isolated from regional lymph node metastases of 59 stage II malignant melanoma patients were analyzed by indirect immunofluorescence staining with monoclonal antibody TAL 1B5, detecting the HLA-DR α chain. The expression of HLA-A,B,C antigens, using antibody W6/32, was also investigated in 45 of these cases. There were no substantial differences in the course of the disease with respect to the percentage of positive cells.

In 13 malignant melanoma patients two to four simultaneous and/or successive metastases (both locoregional and distant-subcutaneous) were analysed for HLA-DR. With simultaneous metastases (7 cases) the percentage of HLA-DR-positive cells was mostly very similar, and in no case was there more than 25% variation. As to successive metastases (9 cases) the percentage of HLA-DR-positive cells remained practically unchanged or decreased during the course of the disease.


Lymph Node Melanoma Monoclonal Antibody Lymph Node Metastasis Cancer Research 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Marta Munzarová
    • 1
  • Daniela Zemanová
    • 1
  • Aleš Rejthar
    • 1
  • Zdenek Mechl
    • 1
  • Věra Kolcová
    • 1
  1. 1.Institute of Medical ResearchResearch Institute of Clinical and Experimental OncologyBrnoCzechoslovakia

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