Journal of tissue culture methods

, Volume 7, Issue 1, pp 1–3

Preparation of low calcium growth medium suitable for determination of tumorigenicity of cultured cells

  • Sabine H. H. Swierenga
  • John P. MacManus


A simplified method for preparation of calcium-deficient growth medium without the use of EGTA is provided. The medium may be used for assessing tumorigenicity of cultured cells.

Key words

low calcium medium cell transformation tumorigenicity 


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Copyright information

© Tissue Culture Association, Inc. 1982

Authors and Affiliations

  • Sabine H. H. Swierenga
    • 1
  • John P. MacManus
    • 2
  1. 1.Drug Toxicology DivisionHealth and Welfare CanadaOttawaCanada
  2. 2.Biological SciencesNational Research Council of CanadaOttawaCanada

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