Journal of tissue culture methods

, Volume 7, Issue 1, pp 1–3

Preparation of low calcium growth medium suitable for determination of tumorigenicity of cultured cells

  • Sabine H. H. Swierenga
  • John P. MacManus
Article

Summary

A simplified method for preparation of calcium-deficient growth medium without the use of EGTA is provided. The medium may be used for assessing tumorigenicity of cultured cells.

Key words

low calcium medium cell transformation tumorigenicity 

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References

  1. 1.
    Swierenga, S. H. H.; Whitfield, J. F.; Karasaki, S. Loss of proliferative calcium dependence: Simplein vitro indicator of tumorigenicity. Proc. Natl. Acad. Sci. USA 75: 6069–6072; 1978.Google Scholar
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    Swierenga, S. H. H.; Whitfield, J. F.; Boynton, A. L.; MacManus, J. P.; Rixon, R. H.; Sikorska, M.; Tsang, B. K.; Walker, P. R. Regulation of proliferation of normal and neoplastic rat liver cells by calcium and cyclic AMP. Ann. N.Y. Acad. Sci. U.S.A. 349: 294–311; 1980.Google Scholar
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    Boyton, A. L.; Swierenga, S. H. H.; Whitfield, J. F. The calcium independence of neoplastic cell proliferation: a promising tool for carcinogen detection. Stich, H. F.; San, R. H. C., eds. Short term tests for chemical carcinogens. New York: Springer Verlag; 1981: 362–371.Google Scholar
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    Swierenga, S. H. H.; Whitfield, J. F.; Gillan, D. J. Alteration by malignant transformation of calcium requirements for cell proliferationin vitro. J. Natl. Cancer Inst. 57: 125–129; 1976.Google Scholar
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    Boynton, A. L.; Whitfield, J. F. Different calcium requirements for proliferation of conditionally and unconditionally tumorigenic mouse cells. Proc. Natl. Acad. Sci. U.S.A. 73: 1651–1654; 1976.Google Scholar

Copyright information

© Tissue Culture Association, Inc. 1982

Authors and Affiliations

  • Sabine H. H. Swierenga
    • 1
  • John P. MacManus
    • 2
  1. 1.Drug Toxicology DivisionHealth and Welfare CanadaOttawaCanada
  2. 2.Biological SciencesNational Research Council of CanadaOttawaCanada

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