Advertisement

Protoplasma

, Volume 156, Issue 1–2, pp 67–73 | Cite as

Detection of hemicelluloses specific to the cell wall of tracheary elements and phloem cells by fluorescein-conjugated lectins

  • T. Hogetsu
Article

Summary

Binding of fluorescein-conjugated wheat-germ agglutinin (F-WGA) and some other lectins to tissues from various plants were examined by epifluorescence microscopy. F-WGA bound specifically to the walls of tracheary elements (TEs) and phloem cells of pea roots. The binding sites in TEs were localized only in the secondary thickening and became evident at very early stages of differentiation. Fluorescein-conjugated derivatives ofSolanum tuberosum lectin,Lycopersicon esculentum lectin, andDatura stramonium lectin, which bind N-acetylglucosamine residues as WGA, also bound to the secondary thickening of TEs of pea roots. The binding sites for F-WGA were not removed by extraction with hot EDTA and proteinase K, but removed by extraction with an alkali solution. The alkali-extracted binding sites from the roots were precipitated together with hemicelluloses by 80% ethanol. These results indicate that the binding sites are not present on pectins, proteins, or cellulose, but hemicelluloses. Localized distribution of the binding sites for F-WGA in TEs was found also in a variety of angiosperm plants.

Keywords

Cell wall Fluorescein-conjugated wheat-germ agglutinin Hemicellulose Lectin Pisum sativum Tracheary element 

Abbreviations

BSL-II

Bandeiraea simplicifolia lectin II

DSL

Datura stramonium lectin

F

fluorescein-conjugated

LEL

Lycopersicon esculentum lectin

MT

microtubule

STL

Solanum tuberosum lectin

TE

tracheary element

WGA

wheat-germ agglutinin

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen AK, Neuberger A (1973) The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein. Biochem J 135: 307–314Google Scholar
  2. — —, Sharon N (1973) The purification, composition and specificity of wheat-germ agglutinin. Biochem J 131: 155–162Google Scholar
  3. Hepler PK, Newcomb EH (1964) Microtubules and fibrils in the cytoplasm ofColeus cells undergoing secondary wall deposition. J Cell Biol 20: 529–533Google Scholar
  4. Herth W (1985) Plasma membrane rosettes involved in localized wall thickening during xylem vessel formation ofLepidium sativum L. Planta 164: 12–21Google Scholar
  5. Hogetsu T (1983) Distribution and local activity of particle complexes synthesizing cellulose microfibrils in the plasma membrane ofClosterium acerosum (Schrank) Ehrenberg. Plant Cell Physiol 24: 777–781Google Scholar
  6. —, Oshima Y (1986) Immunofluorescence microscopy of microtubule arrangement in root cells ofPisum sativum L. var. Alaska. Plant Cell Physiol 27: 939–945Google Scholar
  7. —, Takeuchi Y (1982) Temporal and spatial changes of cellulose synthesis inClosterium acerosum (Schrank) Ehrenberg during cell growth. Planta 154: 426–434Google Scholar
  8. Ingold E, Sugiyama M, Komamine A (1988) Secondary cell wall formation: changes in cell wall constituents during the differentiation of isolated mesophyll cells ofZinnia elegans to TEs. Plant Cell Physiol 29: 295–303Google Scholar
  9. Iyer PNS, Wilkinson KD, Goldstein IJ (1978) AnN-acetyl-D-glucosamine binding lectin fromBandeiraea simplicifolia seeds. Arch Biochem Biophys 177: 330–333Google Scholar
  10. Keller B, Templeton MD, Lamb CJ (1989) Specific localization of a plant cell wall glycine-rich protein in protoxylem cells of the vascular system Proc Natl Acad Sci USA 86: 1529–1533Google Scholar
  11. Kilpatrick DC (1980) Purification and some properties of a lectin from the fruit juice of the tomato (Lycopersicon esculentum). Biochem J 185: 269–272Google Scholar
  12. —, Yeoman MM (1978) Purification of the lectin fromDatura stramonium. Biochem J 175: 1151–1153Google Scholar
  13. Northcote DH (1963) The biology and chemistry of the cell walls of higher plants, algae and fungi. Int Rev Cytol 14: 223–265Google Scholar
  14. - Davey R, Lay J (1989) Use of antisera to localize callose, xylan and arabinogalactan in the cell-plate, primary and secondary walls of plant cells. Planta 178–353–366Google Scholar
  15. Osborn M, Weber K (1982) Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulincontaining structures. In: Wilson L (ed) Methods in cell biology, vol 24. Academic Press, New York, pp 97–132Google Scholar
  16. Pickett-Heaps JC (1967) The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation and distribution of cytoplasmic microtubules. Dev Biol 15: 206–236Google Scholar
  17. Schneider B, Herth W (1986) Distribution of plasma membrane rosettes and kinetics of cellulose formation in xylem development of higher plants. Protoplasma 131: 142–152Google Scholar
  18. Thornber JP, Northcote DH (1961) Changes in the chemical composition of a cambial cell during its differentiation into xylem and phloem tissue in trees. I. Main components. Biochem J 81: 449–455Google Scholar
  19. — — (1961) Changes in the chemical composition of a cambial cell during its differentiation into xylem and phloem tissue in trees. II. Carbohydrate constituents of each main component. Biochem J 81: 455–464Google Scholar
  20. — — (1962) Changes in the chemical composition of a cambial cell during its differentiation into xylem and phloem tissue in trees. III. Xylan, glucomannan and α-cellulose fractions. Biochem J 82: 340–346Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • T. Hogetsu
    • 1
  1. 1.Department of Pure and Applied Sciences, College of Arts and SciencesUniversity of TokyoTokyo

Personalised recommendations