1. In 0.1 ml of bleeding sap of uninoculated plants aspartic acid was found to occur as the sole amino-acid usually in a small concentration. With larger quantities of sap we also found a spot in the chromatograms and in the electropherogram, that most probably is identical with threonine.
2. Under the same conditions with a bleeding period of 4–7 hrs. aspartic acid was the only amino-acid detectable in the chromatogram of the bleeding sap of plants inoculated with ineffective strains.
3. Sap of plants inoculated with effective strains contains aspartic acid asparagine, glutamine, hydroxyproline and ‘threonine’. Sometimes in addition to these substances 1 or 2 unknown substances were found, havingR f-phenol values about identical with those of valine and leucine.
4. Glutamic acid never was detected in the bleeding sap. We suggest, that glutamic acid formed in the nodules might be rapidly transformed into glutamine and transported in this form.
5. The uptake of water is significantly higher with effective strains than with ineffective ones or with plants that have not been inoculated.
6. With this technique it is possible to select effective strains within 3 weeks after planting.
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Wieringa, K.T., Bakhuis, J.A. Chromatography as a means of selecting effective strains ofRhizobia . Plant Soil 8, 254–262 (1957). https://doi.org/10.1007/BF01666160
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