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Establishment of epithelial cell lines from individual rabbit lenses

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We describe a method of initiating primary cell cultures from lenses of rabbits by an explant-outgrowth technique. These primary cultures routinely yield epithelial cell lines when subcultured with collagenase-trypsin chicken serum. Most lines examined retain the diploid chromosome number through at least 30 passages.

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V. References

  1. 1.

    Bloemendal, H. The vertebrate eye lens. Science 197: 127–138; 1977.

  2. 2.

    Papaconstantinou, J. Molecular aspects of lens cell differentiation. Science 156: 338–346; 1967.

  3. 3.

    Harding, C. V.; Reddan, J. R.; Unakar, N. J.; Bagchi, M. The control of cell division in the ocular lens. Int. Rev. Cytol. 31: 215–300; 1971.

  4. 4.

    Reddan, J. R. Development and structure of the lens. Bellows, J. ed. Cataract and abnormalities of the lens. New York City: Grune and Stratton, Inc.; 1974: 29–42.

  5. 5.

    Reddan, J. R.; Unakar, N. J.; Harding, C. V.; Bagchi, M.; Saldana, G. Induction of mitosis in the cultured rabbit lens initiated by the addition of insulin to medium KEI-4. Exp. Eye Res. 20: 45–61; 1975.

  6. 6.

    Reddan, J. R.; Weinsieder, A.; Wilson, D. Aqueous humor from traumatized eyes triggers cell division in the epithelia of cultured lenses. Exp. Eye Res. 28: 267–276; 1979.

  7. 7.

    Reddan, J. R.; Friedman, T. B.; Mostafapour, M. K.; Bondy, R. L.; Sutherland, S. H.; McGee, S. J.; Goldenberg, E. M. Donor age influences the growth of rabbit lens epithelial cellsin vitro. Vision Research 21: 11–23; 1981.

  8. 8.

    Rothstein, H. Experimental techniques for investigation of the amphibian lens epithelium. Prescott, D. M. ed. Methods in cell physiology, vol. 3. New York City: Plenum Press; 1968: 45–74.

  9. 9.

    Tamura, S. Long-term cultures of epithelial cells of a rabbit lens. Jpn. J. Ophthalmol. 9: 177–181; 1965.

  10. 10.

    Shapiro, A. L.; Siegel, I. M.; Scharff, M. D.; Robbins, E. Characteristics of cultured lens epithelium. Invest. Ophthalmol. 8: 393–400; 1969.

  11. 11.

    Coon, H. G. Clonal stability and phenotypic expression of chick cartilage cellsin vitro. Proc. Natl. Acad. Sci. USA 55: 66–73; 1966.

  12. 12.

    Reddan, J. R.; Friedman, T. B.; Bondy, R. L. Influence of donor age on the growth and morphology of lens epithelium in tissue culture (abstr.). J. Cell Biol. CU-072; 1977.

  13. 13.

    Reddan, J. R.; McGee, S. J.; Mostafapour, K. Relationship between donor age and growth potential of mammalian lens epithelial cells (abstr.). J. Cell Biol. CU-437; 1979.

  14. 14.

    Reddan, J. R.; Harding, C. V.; Rothstein, H.; Crotty, M. W.; Lee, P.; Freeman, N. Stimulation of mitosis in the vertebrate lens in the presence of insulin. Ophthalmic Res. 3: 65–82; 1972.

  15. 15.

    Reddan, J. R.; Wilson, D. C. Insulin growth factors I and II stimulate mitosis in the epithelia of mammalian lenses cultured in a serum-free medium (abstr.). J. Cell Biol. CU-469; 1979.

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Reddan, J.R., Friedman, T.B., Mostafapour, M.K. et al. Establishment of epithelial cell lines from individual rabbit lenses. Journal of Tissue Culture Methods 6, 57–60 (1980). https://doi.org/10.1007/BF01666017

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Key words

  • rabbit
  • lens epithelium
  • primary culture
  • cell line
  • lens crystallins