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Establishment of epithelial cell lines from individual rabbit lenses

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Summary

We describe a method of initiating primary cell cultures from lenses of rabbits by an explant-outgrowth technique. These primary cultures routinely yield epithelial cell lines when subcultured with collagenase-trypsin chicken serum. Most lines examined retain the diploid chromosome number through at least 30 passages.

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Reddan, J.R., Friedman, T.B., Mostafapour, M.K. et al. Establishment of epithelial cell lines from individual rabbit lenses. Journal of Tissue Culture Methods 6, 57–60 (1980). https://doi.org/10.1007/BF01666017

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Key words

  • rabbit
  • lens epithelium
  • primary culture
  • cell line
  • lens crystallins