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A method for processing free-floating cultured cells for scanning electron microscopy

Summary

A simple and convenient method is described to aid the processing of minute tissue specimens and isolated single cells for scanning electron microscopy (SEM). The technique employs an inexpensive, disposable carrier fashioned from common Beem capsules and Nitex nylon screen. This method avoids excessive handling, transfers or centrifugation steps that may damage delicate specimens. Specimens processed by this method show excellent preservation of cell surface fine structure.

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McAteer, J.A., Evan, A.P. & Douglas, W.H.J. A method for processing free-floating cultured cells for scanning electron microscopy. Journal of Tissue Culture Methods 7, 85–88 (1982). https://doi.org/10.1007/BF01665947

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Key words

  • specimen processing
  • scanning electron microscopy
  • aggregate cultures
  • isolated cells