Journal of tissue culture methods

, Volume 9, Issue 3, pp 175–177 | Cite as

Cloning hybridoma cells by limiting dilution

  • Joan C. Rener
  • Bruce L. Brown
  • Roland M. Nardone


A method is described for cloning hybridoma cells by limiting dilution. This technique utilizes a feeder layer of murine splenocytes to enhance the growth of clones.

Key words

hybridoma cells cloning feeder layer 


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V. References

  1. 1.
    McKearn, T. J. Cloning of hybridoma cells by limiting dilution in fluid phase. In: Kennett, R. H.; McKearn, T. J.; Bechtol. K. B., eds. Monoclonal antibodies. Hybridomas: a new dimension in biological analyses. New York: Plenum Press; 1980:374.Google Scholar
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    Phillips, H. J. Dye exelusion test for cell viability. In: Kruse. P. F.: Patterson, M. D. Jr., eds. Tissue culture methods and applications. New York: Academic Press; 1973:406–408.Google Scholar
  3. 3.
    Snedecor, G. W. Binomfial and Poisson distribution. Statistical methods. Ames. Iowa: The Iowa State College Press. 1956:482–488.Google Scholar

Copyright information

© Tissue Culture Association, Inc. 1986

Authors and Affiliations

  • Joan C. Rener
    • 1
  • Bruce L. Brown
    • 1
  • Roland M. Nardone
    • 2
  1. 1.Hazleton Biotechnologies CorporationViennaUSA
  2. 2.Center for Advanced Training in Cellular and Molecular BiologyThe Catholic University of AmericaWashington, DCUSA

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