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Establishment of adult syrian golden hamster duodenal epithelial cell cultures from organ explants

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A method is provided for the isolation of duodenal cells from adult Syrian golden hamster duodenum maintained as long-term organ explant cultures. The epithelial cells that grow into a collagen matrix are digested and plated on plastic dishes.

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Contribution No. 1798 of the Cellular Pathoiology Laboratory, University of Maryland.

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Resau, J.H., He', A. & Jones, R.T. Establishment of adult syrian golden hamster duodenal epithelial cell cultures from organ explants. Journal of Tissue Culture Methods 8, 181–184 (1983). https://doi.org/10.1007/BF01665883

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Key words

  • duodenum
  • cell culture
  • organ explant culture
  • Syrian golden hamster