Short-term cultures containing bone marrow mononuclear cells from multiple myeloma patients secrete monoclonal immunoglobulin and β2-microglobulin into the supernatant, which can be measured quantitatively in an enzyme-linked immunosorbant assay. In this system, the addition of interleukin-2 was shown to induce tumor cell regression in the cultures from 10 out of 14 multiple myeloma patients in a dose-dependent manner. Marker analyses of culture cell populations indicate that OKT3 antibody or interleukin-2 did not directly act on the malignant clone but augmented autologous T lymphocytes, which were responsible for the regression of tumor cells in the cultures.
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Peest, D., de Vries, I., Hölscher, R. et al. Effect of interleukin-2 on the ex vivo growth of human myeloma cells. Cancer Immunol Immunother 30, 227–232 (1989). https://doi.org/10.1007/BF01665009
- Tumor Cell
- Bone Marrow
- Mononuclear Cell
- Multiple Myeloma