Infection

, Volume 10, Issue 1, pp 5–8

Subacute and chronic meningoencephalitis in mice after experimental infection with a strain of Naegleria fowleri originally isolated from a patient

  • S. Dempe
  • K. Janitschke
  • A. J. Martinez
Originalia

Summary

Swiss mice were inoculated intranasally with trophozoites of a cloned substrain of theNaegleria fowleri strain LL originally isolated from a human being. The original strain had decreased in virulence after ten years of maintenance in axenic culture. Survivors were sacrificed from the fourth to the tenth week p. i. They were tested in a labyrinth experiment in which some demonstrated a diminution in performance.N. fowleri could be reisolated from the brains of five clinically inconspicuous animals. The histopathological findings in the brains of these animals resembled the features of a chronic granulomatous amebic encephalitis (GAE) which has been described in infections due toAcanthamoeba spp. It was not expected thatN. fowleri could also produce latent infections. The significance of these findings is discussed.

Subakute und chronische Meningoencephalitis bei Mäusen nach experimenteller Infektion mit einem aus einem Patienten isolierten Naegleria fowleri-Stamm

Zusammenfassung

Swiss-Mäuse wurden mit Trophozoiten eines geklonten Substammes vonNaegleria fowleri (LL) intranasal infiziert. Er war aus einem Patienten isoliert worden und hatte bei zehnjähriger axenischer Kultur seine Virulenz für Mäuse verloren. Von der vierten bis zur zehnten Woche p. i. wurden die überlebenden Versuchstiere getötet und in einem Irrgartentest geprüft. Einzelne Tiere zeigten eine verlängerte Reaktionszeit. Aus fünf Hirnen unauffälliger Mäuse ließ sichN. fowleri rückisolieren. Die histopathologischen Befunde in den Hirnen dieser Tiere ähnelten den Charakteristika einer chronisch-granulomatösen Amöbenenzephalitis, die bei Infektionen durchAcanthamoeba spp. bekannt ist. Es war unerwartet, daßN. fowleri auch zu latenten Infektionen führen kann. Die Bedeutung dieser Befunde wird diskutiert.

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Copyright information

© MMW Medizin Verlag 1982

Authors and Affiliations

  • S. Dempe
    • 1
  • K. Janitschke
    • 1
  • A. J. Martinez
    • 2
  1. 1.Robert Koch-Institut des BundesgesundheitsamtesBerlin 65
  2. 2.Department of PathologyUniversity of Pittsburgh, School of Medicine and Presbyterian-University HospitalPittsburghUSA

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