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Presence of oestrogen receptors on target cells and antiproliferative activity of estramustine phosphate: Positive correlation for human tumours in vitro

  • J. Pavelić
  • I. Zgradić
  • K. Pavelić
Original Papers Experimental Oncology

Summary

Incubation with estramustine phosphate for 24 h inhibited DNA, RNA and protein synthesis in primary cultures of human kidney, mammary, prostatic, cervical and endometrial carcinoma. Not only the presence, but also the concentration of oestrogen receptors correlated with estramustine phosphate effects on tumour cell proliferation.

Key words

Oestrogen receptors Estramustine phosphate Human tumours 

Abbreviations

ER

oestrogen receptor

EMP

estramustine phosphate

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References

  1. Andersson L, Edsmyr F, Jonsson G, Konyves I (1976) Estramustine phosphate therapy in carcinoma of the prostate. Recent Results Cancer Res 60:93Google Scholar
  2. Bjork P, Forsgren B, Gustafsson JA, Pousette A, Hogberg B (1982) Partial characterization and “quantitation” of human prostatic estramustine binding protein. Cancer Res 42:1935Google Scholar
  3. Bradley LM (1980) Mitogen-induced responses. In: Mishell BB, Shiigi SM (eds) Selected methods in cellular immunology. Freeman: San Francisco, p 156Google Scholar
  4. Buchanan LJ, Riches AC (1987) Testosterone-induced DNA synthesis in cultured rat ventral prostate. Effects of estracyt and its derivates. Br J Cancer 55:47Google Scholar
  5. Bulbul MA, Pavelić K, Slocum HK, Frankfurt OS, Rustum YM, Huben RP, Bernacki RJ (1986) Growth of human urologic tumors on extracellular matrix. J Urol 136:512Google Scholar
  6. Forsgren B, Bjork P, Carlstrom K, Gustafsson JA, Pousette A, Hogberg B (1979) Purification and distribution of a major protein in the rat prostate that binds estramustine, a nitrogen mustard derivate of estradiol-17Β. Proc Natl Acad Sci USA 76:3149Google Scholar
  7. Frankfurt OS, Slocum HK, Rustum YM, Arbuck SG, Pavelić ZP, Petrelli N, Huben RP, Pontes EJ, Greco WR (1984) Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. Cytometry 5:7Google Scholar
  8. Fredholm B, Jensen G, Lindskog M, Muntzing J (1974) Effects of estramustine phosphate on growth of DMBA-induced mammary tumors in rats. Acta Pharmacol. Toxicol 35 [Suppl 1]:28Google Scholar
  9. Hansenson M, Lundh B, Hartey-Asp B, Pousette A (1988) Growth inhibiting effects of estramustine on two prostatic carcinoma cell lines, LnCaP and LNCaP-r. Urol Res 16:357Google Scholar
  10. Hartley-Asp B (1984) Estramustine-induced mitotic arrest in two human prostatic carcinoma cell lines DU 145 and PC-3. Prostate 5:93Google Scholar
  11. Hartley-Asp B, Gunnarsson PO (1982) Growth and cell survival following treatment with estramustine, nor-nitrogen mustard, estradiol and testosterone of a human prostatic cancer cell line (Du 145). J Urol 127:818Google Scholar
  12. Hartley-Asp B, Kruse E (1986) Nuclear protein matrix as a target for estramustine-induced cell death. Prostate 9:395Google Scholar
  13. Heyns W (1977) Immunochemical measurements of prostatic binding protein. FEBS Lett 81:43Google Scholar
  14. Kruse E, Johansson S-A, Hartley-Asp B, Gunnarsson RO (1988) Distribution and metabolism of estramustine in HeLa cells and the human prostatic tumour cell line 1013L. Biochem Pharmacol 37:3161Google Scholar
  15. Lea O, Petrusz P, French F (1979) Prostatein. A major secretory protein of the rat ventral prostate. J Biol Chem 254:6196Google Scholar
  16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265Google Scholar
  17. Mittelman A, Shukla SK, Murphy GP (1976) Extended therapy of stage D carcinoma of the prostate with oral estramustine phosphate. J Urol 115:409Google Scholar
  18. Muntzing J, Kirdani RY, Saroff J, Murphy GP, Sandberg AA (1977) Inhibitory effects of estracyt on R-3327 rat prostatic carcinoma. Urology 10:439Google Scholar
  19. Muntzing J, Jensen G, Hogberg B (1979) Pilot study on the growth inhibition by estramustine phosphate (Estracyt) of rat mammary tumours sensitive and insensitive to estrogen. Acta Pharmacol Toxicol 44:1Google Scholar
  20. Nagel R, Kolln CP (1977) Treatment of advanced carcinoma of the prostate with estramustine phosphate. Br J Urol 49:73Google Scholar
  21. Pavelić K, Zgradić I, Osmak M, Popović M (1985) Estramustine phosphate reduced proliferation of murine and human cell lines and murine transplantable tumors. Res Exp Med 185:233Google Scholar
  22. Pavelić K, Bulbul MA, Slocum HK, Pavelić ZP, Rustum YM, Niedbala MJ, Bernacki RJ (1986) Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary human tumors expiants. Cancer Res 46:3653Google Scholar
  23. Pousette A, Bjork P, Forsgren B, Carlstrom K (1987) Mouse monoclonal antibodies against rat estramustine binding protein. J Steroid Biochem 26:509Google Scholar
  24. Pousette A, Bjork P, Carlstrom K, Forsgren B, Gustafsson JA, Hogbers B (1981) Influence of sex hormones on prostatic secretion protein, a major protein in rat prostate. Cancer Res 41:688Google Scholar
  25. Rupp WD, Howard-Flanders P (1968) Discontinuities in the DNA synthesized in an excision-defective strain ofEscherichia coli following ultraviolet irradiation. J Mol Biol 31:291Google Scholar
  26. Shyr C, Liao S (1979) Protein factor that inhibits binding and promotes release of androgen-receptor complex from nuclear chromatin. Proc Natl Acad Sci USA 75:5969Google Scholar
  27. Slocum HK, Pavelić ZP, Rustum YM, Creaven PJ, Karakousis C, Takita H, Greco WR (1981) Characterization of cells obtained by mechanical and enzymatic means from human melanoma, sarcoma, and lung tumors. Cancer Res 41:1428Google Scholar
  28. Wakisaka M, Iwasaki I, Shimazaki J (1979) Effects of estramustine phosphate (Estracyt) on transplantable mouse tumours. Urol Res 7:291Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. Pavelić
    • 1
  • I. Zgradić
    • 1
  • K. Pavelić
    • 1
  1. 1.Ruter Bošković InstituteZagrebYugoslavia

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