Virchows Archiv A

, Volume 421, Issue 2, pp 157–161 | Cite as

Keratin profiles in normal/hyperplastic prostates and prostate carcinoma

  • Hiyoshi Okada
  • Airo Tsubura
  • Akiharu Okamura
  • Hideto Senzaki
  • Yuji Naka
  • Yosuke Komatz
  • Sotokichi Morii
Original Articles
Received:
Accepted:

Summary

Immunoreactivities in 25 cases of prostatic adenocarcinoma and 10 normal/hyperplastic prostates were investigated in methacarn-fixed, paraffin-embedded serial sections using a panel of nine anti-keratin monoclonal antibodies (mAbs); 34β E12, CK8.12, 312C8-1, CK4.62, RPN1165, RPN1162, 35βH11, CK5, M20, and one of anti-actin mAb, HHF35. In normal/ hyperplastic prostates, RPN1162, 35βH11, CK5 and M20 stained luminal cells without staining basal cells, and 34βE12, CK8.12 and 312C8-1 stained basal cells but not luminal cells. Other mAbs, CK4.62 and RPN1165, stained basal cells as well as luminal cells. All of the mAbs labelling luminal cells stained cancer cells with variable frequencies in a manner unrelated to the grade of tumour differentiation. Of the prostate cancer cases 92% were scored positive with M20, 84% with 35βH11, 80% with CK5, 68% with CK4.62, 60% with RPN1165 and 4% with RPN1162. However, basal cell-specific keratins labelled with 34βE12, CK8.12 and 312C8-1 were totally negative in the cancer cells. HHF35 showed no labelling in normal, hyperplastic or neoplastic epithelial cells of the prostate. Our findings indicate that the major part of the cells of prostatic adenocarcinomas have keratin phenotypes similar to luminal cells but not basal cells, and that no myoepithelial differentiation can be detected in epithelial cell of the prostate. Thus, mAbs for keratins facilitate the identification of epithelial cell phenotypes in normal, benign and malignant conditions of the prostate.

Key words

Prostate cancer Luminal cell Basal cell Keratin Actin 
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References

  1. Achtstätter T, Moll R, Moore B, Franke WW (1985) Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract: immunofluorescence and gel electrophoretic studies. J Histochem Cytochem 33:415–426Google Scholar
  2. Aumüller G, Gröschel-Stewart U, Altmannsberger M, Mannherz HG, Steinhoff M (1991) Basal cells of H-Dunning tumor are myoepithelial cells. A comparative immunohistochemical and ultrastructural study with male accessory sex glands and mammary gland. Histochemistry 95:341–349Google Scholar
  3. Barwick KW, Mardi JA (1983) An immunohistochemical study of the myoepithelial cell in prostatic hyperplasia and neoplasia. Lab Invest 48:7AGoogle Scholar
  4. Bostwick DG, Brawer MK (1987) Prostatic intra-epithelial neoplasia and early invasion in prostate cancer. Cancer 59:788–794Google Scholar
  5. Brawer MK, Peehl DM, Stamey TA, Bostwick DG (1985) Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 45:3663–3667Google Scholar
  6. Dairkee SH, Blayney C, Smith HS, Hackett AJ (1985) Monoclonal antibody that defines human myoepithelium. Proc Natl Acad Sci USA 82:7409–7413Google Scholar
  7. Dairkee SH, Puett L, Hackett AJ (1988) Expression of basal and luminal epithelium-specific keratins in normal benign and malignant breast tissue. J Natl Cancer Inst 80:691–695Google Scholar
  8. Gleason DF (1977) Histological grading and clinical staging of prostatic carcinoma. In: Tannenbaum M (ed) Urologic pathology, the prostate. Lea and Febiger, Philadelphia, pp 171–197Google Scholar
  9. Gown AM, Vogel AM (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am J Pathol 114:309–321Google Scholar
  10. Grignon DJ, Ro JY, Ordoñez NG, Ayala AG, Cleary KR (1988) Basal cell hyperplasia, adenoid basal cell tumor, and adenoid cystic carcinoma of the prostate gland: an immunohistochemical study. Hum Pathol 19:1425–1433Google Scholar
  11. Guinan P, Shaw M, Targonski P, Ray V, Rubenstein M (1989) Evaluation of cytokeratin markers to differentiate between benign and malignant prostatic tissue. J Surg Oncol 42:175–180Google Scholar
  12. Hedrick L, Epstein JI (1989) Use of keratin 903 as adjunct in the diagnosis of prostate carcinoma. Am J Pathol 13:389–396Google Scholar
  13. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24Google Scholar
  14. Moll R, Krepler R, Franke WW (1983) Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation 23:256–269Google Scholar
  15. Mostofi FK, Sesterhenn I, Sobin LH (1980) Histological typing of the prostate tumors. International histological classification of tumors, no. 22. WHO, GenevaGoogle Scholar
  16. Nagle RB, Ahmann FR, McDaniel KM, Paquin ML, Clark VA, Celniker A (1987) Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Res 47:281–286Google Scholar
  17. Nagle RB, Brawer MK, Kittelson J, Clark V (1991) Phenotypic relationships of prostatic intraepithelial neoplasia to invasive prostatic carcinoma. Am J Pathol 138:119–128Google Scholar
  18. O'Mallery FP, Grignon DJ, Shum DT (1990) Useful of immunoperoxidase staining with high-molecular-weight cytokeratin in differential diagnosis of small-acinar lesions of the prostate gland. Virchows Arch [A] 417:191–196Google Scholar
  19. Purnell DM, Heatfield BM, Anthony RL, Trump BF (1987) Immunohistochemistry of the cytoskeleton of human prostatic epithelium. Evidence for disturbed organization in neoplasia. Am J Pathol 126:384–395Google Scholar
  20. Ramaekers FCS, Puts JJG, Moesker O, Kant A, Huysmans A, Haag D, Jap PHK, Herman CJ, Vooijs GP (1983) Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumors: an overview. Histochem J 15:691–713Google Scholar
  21. Senzaki H, Tsubura A, Shoji T, Okada H, Takahashi H, Morii S (1992) Immunohistochemical detection of keratin, actin and type IV collagen in serial sections of methacarn-fixed breast cancer tissue. Acta Histochem Cytochem 25:31–38Google Scholar
  22. Shah IA, Schlageter M-O, Stinnett P, Lechago J (1991) Cytokeratin immunohistochemistry as a diagnostic tool for distinguishing malignant from benign epithelial lesions of the prostate. Mod Pathol 4:220–224Google Scholar
  23. Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C (1990) Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 143:167–171Google Scholar
  24. Srigley JR, Dardick I, Warren R, Hartwick J, Klotz L (1990) Basal epithelial cells of human prostate gland are not myoepithelial cells. A comparative immunohistochemical and ultrastructural study with the human salivary gland. Am J Pathol 136:957–966Google Scholar
  25. Tsubura A, Shikata N, Inui T, Morii S, Hatano T, Oikawa T, Matsuzawa A (1988) Immunohistochemical localization of myoepithelial cells and basement membrane in normal, benign and malignant human breast lesions. Virchows Arch [A] 413:133–139Google Scholar
  26. Tsubura A, Inui T, Senzaki H, Morii S, Dairkee SH (1989) Immunolocalization of the human basal epithelial marker monoclonal antibody 312C8-1 in normal tissue and mammary tumours of rodents. Virchows Arch [A] 415:533–538Google Scholar
  27. Tsubura A, Hatano T, Hayama S, Morii S (1991 a) Immunopheno-typic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat 140:287–293Google Scholar
  28. Tsubura A, Okada H, Sasaki M, Dairkee SH, Morii S (1991 b) Immunohistochemical demonstration of keratin 8 and 14 in benign tumors of the skin appendage. Virchows Arch [A] 418:503–507Google Scholar
  29. Tsubura A, Okada H, Senzaki H, Hatano T, Morii S (1991 c) Keratin expression in the normal breast and in breast carcinoma. Histopathology 18:517–522Google Scholar
  30. Tsukada T, McNutt MA, Ross R, Gown AM (1987) HHF35, a muscle actin-specific monoclonal antibody: II. Reactivity in normal, reactive and neoplastic human tissues. Am J Pathol 127:389–402Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Hiyoshi Okada
    • 1
  • Airo Tsubura
    • 2
  • Akiharu Okamura
    • 3
  • Hideto Senzaki
    • 2
  • Yuji Naka
    • 1
  • Yosuke Komatz
    • 1
  • Sotokichi Morii
    • 2
  1. 1.Departments of UrologyKansai Medical UniversityOsakaJapan
  2. 2.Departments of PathologyKansai Medical UniversityOsakaJapan
  3. 3.Departments of Clinical and Laboratory MedicineKansai Medical UniversityOsakaJapan

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