Virchows Archiv A

, Volume 417, Issue 4, pp 333–341 | Cite as

Insulin therapy prevents spontaneous recovery from streptozotocin-induced diabetes in Syrian hamsters

An autoradiographic and immunohistochemical study
  • Parviz M. Pour
  • William Duckworth
  • Kim Carlson
  • Kathy Kazakoff


Streptozotocin (Sz) given as a single dose of 50 mg/kg body wt. caused severe diabetes in Syrian hamsters. However, the level of blood glucose decreased gradually after 21 days post-Sz and reached the near normal level at 70 days in 90% of hamsters. The recovery from diabetes was associated with the regeneration of theβ-cells of islets and a reduction in the initially increased number ofα- andδ-cells. Daily treatment of diabetic hamsters with insulin was associated with the persistence of severe diabetes, lack of or minimal tendency forβ-cell regeneration and sustained hyperplasia ofα- andδ-cells in 90% of hamsters. Insulin also inhibited DNA synthesis (as measured by incorporation of tritiated thymidine), in ductal, ductular and acinar cells in Sz-pretreated hamsters but not in normoglycemic control hamsters treated with insulin alone. The results demonstrate a deleterious effect of exogenous insulin in the course of Sz-induced diabetes in hamsters.

Key words

Streptozotocin Diabetes Syrian hamsters Autoradiography Immunohistochemistry 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baetens D, Stefan Y, Ravazzola M, Malaisse-Lagae F, Coleman DL, Orci L (1978) Alteration of islet cell populations in spontaneously diabetic mice. Diabetes 217:1–7Google Scholar
  2. Bell RH, Strayer DS (1983) Streptozotocin prevents development of nitrosamine-induced pancreatic cancer in the Syrian hamster. J Surg Oncol 24:258Google Scholar
  3. Bendayan M, Grégoire S (1987) Immunohisto- and cytochemical studies of pancreatic enzymes in peri-insular and tele-insular acinar cells of streptozotocin-induced diabetic rats. Pancreas 2:272–282Google Scholar
  4. Brockenbourgh JS, Weir GC, Bonner-Weir S (1988) Discordance of exocrine and endocrine growth after 90% pancreatectomy in rats. Diabetes 37:232Google Scholar
  5. Cantenys D, Portha B, Dutrillaux MC, Hollander E, Roze C, Picon L (1981) Histogenesis of the endocrine pancreas in newborn rats after destruction by streptozotocin. An immunocyto-chemical study. Virchows Arch [B] 35:109–122Google Scholar
  6. Chick WL (1973) Beta cell replication in rat pancreatic monolayercultures: effects of glucose, tolbutamide, glucocorticoid, growth hormone and glucagon. Diabetes 22:687Google Scholar
  7. Dulin WE, Gerritsen GC, Chang AY (1983) Experimental and spontaneous diabetes in animals. In: Rifkin H, Porte D Jr (eds) Diabetes mellitus. Medical Examination Publishing, New York, pp 361–408Google Scholar
  8. Dutrillaux MC, Portha B, Roze C, Hollande E (1982) Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin. Virchows Arch [B] 39:173–185Google Scholar
  9. Gotoh M, Monden M, Okamura J, Mori T, Shima K (1987) Correlation of regional insulin and glucagon content in canine pancreas and a possible interaction of A and B cells. Endocrinol Jpn 34:843–847Google Scholar
  10. Haist RE, Best CH (1940) Factors affecting the insulin content of pancreas. Science 91:410–411Google Scholar
  11. Hashimoto T, Kawano H, Daikoku S, Shima K, Taniguchi H, Baba S (1988) Transient coappearance of glucagon and insulin in the progenitor cells of the rat pancreatic islet. Anat Embryol (Berl) 178:489–497Google Scholar
  12. Hirsch GC (1932) Die Lebendbeobachtung der Restitution im Pankreas, IV. Z Zellforsch Mikrosk Anat 15:290–310Google Scholar
  13. House EL, Tassoni JP (1957) Duration of alloxan diabetes in the hamster. Endocrinology 61:309Google Scholar
  14. Karunanayake EH, Hearse DJ, Mellows G (1976) Streptozotocin: its excretion and metabolism in the rat. Diabetologia 12:483–499Google Scholar
  15. Katsilambros N, Rahman YA, Hinz M, Fussganger R, Schroder K, Straub K, Pfeiffer EF (1970) Action of streptozotocin on insulin and glucagon responses of rat islets. Horm Metab Res 2:268–270Google Scholar
  16. Like AA, Chick WL (1970) Studies in the diabetic mutant mouse: I. Light microscopy and radioautography of pancreatic islets. Diabetologia 6:207–215Google Scholar
  17. Logothetopoulos J, Brodsky G (1968) Mitotic activity of islet cells in alloxan and streptozotocin diabetic mice studied by radioautography. Diabetes [Suppl] 17:306Google Scholar
  18. Logothetopoulos J, Valiquette N, Dvet D (1983) Glucose stimulation of beta cell DNA replication in the intact rat and in pancreatic islets in suspension culture. Diabetes 32:1172Google Scholar
  19. McEvoy RC, Hegre OD (1977) Morphometric quantitation of the pancreatic insulin-, glucagon-, and somatostatin-positive cell populations in normal and alloxan-diabetic rats. Diabetes 26:1140–1146Google Scholar
  20. Patel YC, Weir GC (1976) Increased somatostatin content of islets from streptozotocin-diabetic rats. Clin Endocrinol 51:191–194Google Scholar
  21. Patel YC, Cameron D, Bankier A, Malaisse-Lagae F, Ravazzola M, Studer P, Orci L (1978) Changes in somatostatin concentration in pancreas and other tissues of streptozotocin diabetic rats. Endocrinology 103:917–923Google Scholar
  22. Phares CK (1980) Streptozotocin-induced diabetes in Syrian hamsters: new model of diabetes mellitus. Experientia 36:681Google Scholar
  23. Pour PM (1978) Islet cells as a component of pancreatic ductal neoplasms. I. Experimental study. Ductular cells, including islet cell precursors as primary progenitor cells of tumors. Am J Pathol 90:295–316Google Scholar
  24. Rakieten N, Gordon BS, Beaty A, Cooely DA, Shein PS (1976) Modification of renal tumorigenic affect of streptozotocin by nicotinamide. Proc Soc Exp Biol Med 151:356Google Scholar
  25. Steiner H, Oelz O, Zahnd G, Froesch ER (1970) Studies on islet cell regeneration, hyperplasia and intrainsular cellular interrelations in long-lasting streptozotocin diabetes in rats. Diabetologia 6:558–564Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Parviz M. Pour
    • 1
    • 2
  • William Duckworth
    • 3
  • Kim Carlson
    • 1
  • Kathy Kazakoff
    • 1
  1. 1.The Eppley Institute for Research in CancerUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Department of Pathology and MicrobiologyUniversity of Nebraska Medical CenterOmahaUSA
  3. 3.Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaUSA

Personalised recommendations