Virchows Archiv A

, Volume 417, Issue 4, pp 333–341 | Cite as

Insulin therapy prevents spontaneous recovery from streptozotocin-induced diabetes in Syrian hamsters

An autoradiographic and immunohistochemical study
  • Parviz M. Pour
  • William Duckworth
  • Kim Carlson
  • Kathy Kazakoff
Article

Summary

Streptozotocin (Sz) given as a single dose of 50 mg/kg body wt. caused severe diabetes in Syrian hamsters. However, the level of blood glucose decreased gradually after 21 days post-Sz and reached the near normal level at 70 days in 90% of hamsters. The recovery from diabetes was associated with the regeneration of theβ-cells of islets and a reduction in the initially increased number ofα- andδ-cells. Daily treatment of diabetic hamsters with insulin was associated with the persistence of severe diabetes, lack of or minimal tendency forβ-cell regeneration and sustained hyperplasia ofα- andδ-cells in 90% of hamsters. Insulin also inhibited DNA synthesis (as measured by incorporation of tritiated thymidine), in ductal, ductular and acinar cells in Sz-pretreated hamsters but not in normoglycemic control hamsters treated with insulin alone. The results demonstrate a deleterious effect of exogenous insulin in the course of Sz-induced diabetes in hamsters.

Key words

Streptozotocin Diabetes Syrian hamsters Autoradiography Immunohistochemistry 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Parviz M. Pour
    • 1
    • 2
  • William Duckworth
    • 3
  • Kim Carlson
    • 1
  • Kathy Kazakoff
    • 1
  1. 1.The Eppley Institute for Research in CancerUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Department of Pathology and MicrobiologyUniversity of Nebraska Medical CenterOmahaUSA
  3. 3.Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaUSA

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