Virchows Archiv A

, Volume 418, Issue 1, pp 1–7 | Cite as

Undecalcified preparation of bone tissue: Report of technical experience and development of new methods

  • Michael Hahn
  • Martin Vogel
  • Günter Delling
Article

Summary

For reliable quantitative and qualitative analysis of bone specimens undecalcified preparation is essential. The “conventional” technique for this purpose is embedding in methylmethacrylate. Larger bone specimens, highly sclerotic specimens, cortical bone or bone implants consisting of metals or ceramics require modifications of this technique or completely new methods. We report our experience with the undecalcified preparation of 47700 bone specimens. New techniques such as the cutting of large area sections up to a size of 5×6 cm and grinding procedures for completely artefact-free preparation which are applied in special cases are also described. A new technique of combinded two- and three-dimensional analysis of bone specimens is presented. In our experience these methods are fundamental for morphological investigation of bone.

Key words

Bone Undecalcified preparation Grinding technique 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Michael Hahn
    • 1
  • Martin Vogel
    • 1
  • Günter Delling
    • 1
  1. 1.Department of Bone Pathology, Institute of PathologyUniversity of HamburgHamburg 20Federal Republic of Germany

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