Current Microbiology

, Volume 17, Issue 4, pp 229–234

Glucose utilization and growth response to protein hydrolysates byFusobacterium species

  • Saheer E. Gharbia
  • Haroun N. Shah
Article

DOI: 10.1007/BF01589457

Cite this article as:
Gharbia, S.E. & Shah, H.N. Current Microbiology (1988) 17: 229. doi:10.1007/BF01589457

Abstract

Glucose uptake was measured in the supernatants of 18 strains ofFusobacterium species cultured in BM medium. Some species, such asF. nucleatum andF. necrophorum, used between 25% and 48% of the glucose in the medium, but the terminal pH remained near neutral. By contrast, strains ofF. mortiferum andF. necrogenes used on average over 90% of the available glucose in the medium and produced a predictably low acidic pH. Strains ofF. varium used between 86% and 91% of the glucose present but produced a near neutral pH of between 5.8 and 5.9. The metabolic fate of glucose inF. varium was, therefore, examined in more detail. Glucose stimulated the growth of this species, and [14C]glucose was incorporated into the metabolic end products and various cellular components. Protein hydrolysates, tested for their growth-promoting effects onFusobacterium species, produced two general growth response patterns. Most species grew prolifically on trypticase, proteose peptone, and yeast extract, but poorly in casamino acids and vitamin-free casamino acids. Growth in bactocasitone was poor, but for three species,F. necrophorum, F. varium, andF. nucleatum, there was an approximately linear growth response up to 0.5%. These results suggest a major role for nitrogen metabolism but do not preclude glucose as an energy source in at least some species ofFusobacterium.

Copyright information

© Springer-Verlag New York Inc 1988

Authors and Affiliations

  • Saheer E. Gharbia
    • 1
  • Haroun N. Shah
    • 1
  1. 1.Department of Oral MicrobiologyThe London Hospital Medical CollegeLondonUK

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