Current Microbiology

, Volume 17, Issue 4, pp 203–208 | Cite as

Genetic characterization of cannibalism inDictyostelium caveatum

  • Karen T. I. Duffy
  • David R. Waddell
Article

Abstract

The parasexual cycle has been developed in the predatory slime mold,Dictyostelium caveatum, in order to study phagocytosis and recognition mechanisms. Cannibalistic strains were obtained in which self/non-self recognition mechanisms have broken down. These strains are haploid, but often multinucleate. This phenotype was investigated genetically and was shown to be the result of recessive mutations. Analysis of 14 independently derived cannibal strains indicates that this phenotype maps to at least two complementation groups.

Keywords

Mold Genetic Characterization Recessive Mutation Slime Mold Complementation Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Buxton EW (1956) Heterokaryosis and parasexual recombination inPenicillium chrysogenum. J Gen Microbiol 15:133–139Google Scholar
  2. 2.
    Duffy KTI, Vogel G (1984) Linkage analysis of two phagocytosis receptor loci inDictyostelium discoideum. J Gen Microbiol 130:2071–2077Google Scholar
  3. 3.
    Fincham JRS, Day PR, Radford A (1979) Fungal genetics. Botanical Monographs 4. Oxford, England: Blackwell Scientific PublicationsGoogle Scholar
  4. 4.
    Hastie AC (1964) The parasexual cycle inVerticillium alboatrum. Genet Res Camb 5:305–315Google Scholar
  5. 5.
    Katz ER, Sussman M (1972) Parasexual recombination inDictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants. Proc Natl Acad Sci USA 69:495–498Google Scholar
  6. 6.
    Loomis WF (1969) Temperature sensitive mutants ofDictyostelium discoideum. J Bacteriol 99:65–69Google Scholar
  7. 7.
    Loomis WF, Ashworth JM (1968) Plaque-sized mutants of the cellular slime mouldDictyostelium discoideum. J Gen Microbiol 53:181–186Google Scholar
  8. 8.
    Newell PC (1978) Genetics of the cellular slime moulds. Annu Rev Genet 96:115–123Google Scholar
  9. 9.
    Pontecorvo G, Kafer E (1958) Genetic analysis by means of mitotic recombination. Advan Genet 9:71–104Google Scholar
  10. 10.
    Pontecorvo G, Sermonti G (1954) Parasexual recombination inPenicillium chrysogenum. J Gen Microbiol 11:94–104Google Scholar
  11. 11.
    Sinha U, Ashworth JM (1969) Evidence for the existence of elements of a parasexual cycle in the cellular slime mould,Dictyostelium discoideum. Proc R Soc Edinburgh Sect B 173:531–540Google Scholar
  12. 12.
    Sussman M, Sussman RR (1962) Ploidal inheritance inDictyostelium discoideum I. Stable haploid, stable diploid, and metastable strains. J Gen Microbiol 28:417–429Google Scholar
  13. 13.
    Tinline RD (1962)Cochliobolus sativus. V. Heterokaryosis and parasexuality. Can J Bot 40:425–437Google Scholar
  14. 14.
    Waddell DR (1982) A predatory slime mould. Nature 298:464–466Google Scholar
  15. 15.
    Waddell DR, Duffy KTI (1986) Breakdown of self/non-self recognition in cannibalistic strains of the predatory slime mould,Dictyostelium caveatum. J Cell Biol 102:298–305Google Scholar
  16. 16.
    Waddell DR, Vogel G (1985) Phagocytic behavior of the predatory slime mold,Dictyostelium caveatum. Cell nibbling. Exp Cell Res 159:323–334Google Scholar
  17. 17.
    Waddell DR, Duffy KTI, Vogel G (1987) Cytokinesis is defective inDictyostelium mutants with altered phagocytotic recognition, adhesion, and vegetative cell cohesion properties. J Cell Biol, in pressGoogle Scholar
  18. 18.
    Warren AJ, Warren WD, Cox EC (1976) Genetic and morphological study of aggregation in the cellular slime moldPolysphondylium violaceum. Genetics 83:25–47Google Scholar

Copyright information

© Springer-Verlag New York Inc 1988

Authors and Affiliations

  • Karen T. I. Duffy
    • 1
  • David R. Waddell
    • 1
  1. 1.Division of BiochemistryBergische University-GHSWuppertalFRG

Personalised recommendations