Current Microbiology

, Volume 16, Issue 2, pp 61–67

Inhibitory factors involved in acetone-butanol fermentation byClostridium saccharoperbutylacetonicum

  • Bhupinder K. Soni
  • Kumudeswar Das
  • Tarun K. Ghose


Studies carried out onClostridium saccharoperbutylacetonicum (ATCC 27022) reveal that intracellular and extracellular inhibitors, including metabolic end-products, caused the inhibition of cell growth and solvent production. Butanol at the level of 13.0 g/liter was completely inhibitory to the growth of cells, whereas butyric acid totally inhibited the cell growth at a concentration of 8.7 g/liter. Investigations carried out on the effect of addition of culture filtrate concentrate and cell-free extract concentrate indicate that nonvolatile inhibitors produced by cells were also inhibitory for bioconversion. The butanol production was found to be reduced by 15%–20% on addition of cell-free extract concentrate. Inhibition by concentrates was enhanced in the presence of butanol. Addition of heat-sterilized concentrates resulted in a reduction of inhibition.


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Copyright information

© Springer-Verlag New York Inc 1987

Authors and Affiliations

  • Bhupinder K. Soni
    • 1
  • Kumudeswar Das
    • 1
  • Tarun K. Ghose
    • 1
  1. 1.Biochemical Engineering Research CenterIndian Institute of TechnologyNew DelhiIndia
  2. 2.Department de Genie Biochemique et AlimentaireINSAToulouse, CedexFrance

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