Journal of Industrial Microbiology

, Volume 11, Issue 1, pp 1–6

High production of alkaline protease byBacillus licheniformis in a fed-batch fermentation using a synthetic medium

  • Weiying Mao
  • Renrui Pan
  • David Freedman
Article

DOI: 10.1007/BF01583724

Cite this article as:
Mao, W., Pan, R. & Freedman, D. Journal of Industrial Microbiology (1992) 11: 1. doi:10.1007/BF01583724

Summary

High production (9016 U/ml) of alkaline protease byBacillus licheniformis has been achieved. A 49% increase in production was achieved by the method used as compared with a batch process. By using a synthetic medium and a fed-batch operation controlled by the Advanced Fermentation Software (AFS) package, it was found that the keys to high production of protease are: (i) to maintain a low concentration of glucose (<0.43 g/l) in the medium; (ii) to control pH at a certain level (pH 6.50) in the culture; and (iii) to use rough type colonies as the starting culture. Our fed-batch fermentation process successfully simulates and surpasses ordinary batch fermentation processes. By using ammonium sulfate instead of soy bean flour as the only nitrogen source, an expected benefit was the elimination of unpleasant odors caused by natural organic nitrogenous components in the media. This would improve the industrial production environment.

Key words

Protease Bacillus licheniformis Synthetic medium Fed-batch fermentation 

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Copyright information

© Society for Industrial Microbiology 1992

Authors and Affiliations

  • Weiying Mao
    • 1
  • Renrui Pan
    • 2
  • David Freedman
    • 1
  1. 1.R&D LaboratoryNew Brunswick Scientific Co., Inc.EdisonUSA
  2. 2.Department of BiologyUniversity of Science and Technology of ChinaHefei AnhuiChina

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