Efficacy of commercial inocula in enhancing biodegradation of weathered crude oil contaminating a Prince William Sound beach
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Summary
In a laboratory study evaluating the effectiveness of 10 commercial products in stimulating enhanced biodegradation of Alaska North Slope crude oil, two of the products provided significantly greater alkane degradation in closed flasks than indigenous Alaskan bacterial populations supplied only with excess nutrients. These two products, which were microbial in nature, were then taken to a Prince William Sound beach to determine if similar enhancements were achieveable in the field. A randomized complete block experiment was designed in which four small plots consisting of a no-nutrient control, a mineral nutrient plot, and two plots receiving mineral nutrients plus the two products were laid out in random order on a beach in Prince William Sound that had been contaminated 16 months earlier from the Exxon Valdez spill. These four plots comprised a ‘block’ of treatments, each oil residue weight and alkane hydrocarbon profile changes. The results indicated no significant differences (P<0.05) among the four treatments in the 27-day time period of the experiment. A statistical power analysis, however, revealed that the variability in the data prevented a firm conclusion in this regard. Failure to detect significant differences was attributed not only to variability in the data but also to the highly weathered nature of the oil and the lack of sufficient time for biodegradation to take place.
Key words
Oil biodegradation Allochthonous bacteria Field testing Randomized block Oil spill Alkanes HydrocarbonsPreview
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References
- 1.Atlas, R.M. and R. Bartha. 1973. Abundance, distribution, and oil biodegradation potential of microorganisms in Raritan Bay. Environ. Pollut. 4: 291–300.Google Scholar
- 2.Atlas, R.M., P.D. Boehm and J.A. Calder. 1980. Chemical and biological weathering of oil from theAmoco Cadiz oil spillage in the littoral zone. Estuarine Coastal Mar. Sci. 12: 589–608.Google Scholar
- 3.Boehm, P.D. and D.L. Fiest. 1980. Comparative weathering patterns of hydrocarbons from theAmoco Cadiz oil spill observed at a variety of coastal environments. In: Proc. Int. Symp. on theAmoco Cadiz: fates and effects of the oil spill. Centre Oceanologique de Bretagne, Brest, France.Google Scholar
- 4.Bushnell, L.D. and F.F. Haas. 1941. The utilization of certain hydrocarbons by microorganisms. J. Bacteriol. 41: 653–673.Google Scholar
- 5.Christiansen, J.A. and P.W. Spraker. 1982. Improving effluent quality of petrochemical wastewaters with mutant bacterial cultures; Proc. Purdue Ind. Wastes Conf. 37: 567–576.Google Scholar
- 6.Dott, W., D. Feidieker, P. Kampfer, H. Schleibinger and S. Strechel. 1989. Comparison of autochthonous bacteria and commercially available cultures with respect to their effectiveness in fuel oil degradation. J. Ind. Microbiol. 4: 365–374.Google Scholar
- 7.Colwell, R.R., A.L. Mills, J.D. Walker, P. Garcia-Tello and V. Campos-P. 1978. Microbial ecology of theMetula spill in the Straits of Magellan. J. Fish. Res. Board Can. 35: 573–580.Google Scholar
- 8.Horowitz, A. and R.M. Atlas. 1978. Crude oil degradation in the Artic: changes in bacterial populations and oil composition during one year exposure in a model ecosystem. Dev. Ind. Microbiol. 19: 517–522.Google Scholar
- 9.Horowitz, A., A.J. Sexstone and R.M. Atlas. 1978. Hydrocarbons and microbial activities in sediment of an Artic lake one year after contamination with leaded gasoline. Artic 31: 180–191.Google Scholar
- 10.Leahy, J.G. and R.R. Colwell. 1990. Microbial degradation of hydrocarbons in the environment. Microbiol. Rev. 54: 305–315.Google Scholar
- 11.Lehtomaki, M. and S. Niemela. 1975. Improving microbial degradation of oil in soil. Ambio 4: 126–129.Google Scholar
- 12.Pritchard, P.H. and C.F. Costa. 1991. EPA's Alaska oil spill bioremediation project. Environ. Sci. Techol. 25: 372–379.Google Scholar
- 13.Rashid, M.A. 1974. Degradation of bunker C oil under different coastal environments of Chedabucto Bay, Nova Scotia. Estuarine Coastal Mar. Sci. 2: 137–144.Google Scholar
- 14.Sexstone, A.J. and R.M. Atlas. 1977. Response of populations in artic tundra soils to crude oil. Can. J. Microbiol. 23: 1327–1333.Google Scholar
- 15.Stewart, J.E. and L.J. Marlas. 1978. Distribution and abundance of hydrocarbon-utilizing bacteria in sediments of Chedabucto Bay, Nova Scotia, in 1976. J. Fish. Res. Bd. Can. 35: 581–584.Google Scholar
- 16.U.S. Environmental Protection Agency. 1979. Methods for chemical analysis of water and wastes. EPA 600/4-79-020, U.S. Environmental Protection Agency, Washington, D.C.Google Scholar
- 17.Venosa, A.D., J.R. Haines, W. Nisamaneepong, R. Govind, S. Pradhan and B. Siddique. 1991. Efficacy of commercial inocula in enhancing oil biodegradation in closed laboratory reactors. J. Ind. Microbiol. 10: 13–23.Google Scholar
- 18.Walker, J.D. and R.R. Colwell. 1976. Petroleum: degradation by estuarine microorganism. In: Proc. 3rd Int. Biodegradation Symp. (J.M. Sharpley and A.M. Kaplan (eds.)), Applied Sci. Publishers, Ltd., London.Google Scholar
- 19.Wong, A.D. and C.D. Goldsmith. 1988. The impact of a chemostat discharge containing oil degrading bacteria on the biological kinetics of a refinery activated sludge process. Water Sci. Technol. 20: 131–136.Google Scholar