Origins of life and evolution of the biosphere

, Volume 23, Issue 1, pp 53–64

Comparison of membrane ATPases from extreme halophiles isolated from ancient salt deposits

  • Helga Stan-Lotter
  • Michael Sulzner
  • Eva Egelseer
  • Cynthia F. Norton
  • Lawrence I. Hochstein
Article

Abstract

Halophilic microorganisms were isolated from Triassic and Permian salt deposits. Two were rods and grew as red colonies; another was a coccus and produced pink colonies. The rods lysed in solutions that lacked added sodium chloride. Growth of all isolates was inhibited by aphidicolin and their bulk proteins were acidic as judged from isoelectric focusing. Therefore, these organisms were tentatively identified as extreme halophiles. Whole cell proteins patterns of the isolates following gel electrophoresis were distinct and differed from those of representative type strains of halophilic bacteria. The membrane ATPases from the rods were similar to the enzyme fromHalobacterium saccharovorum with respect to subunit composition, enzymatic properties and immunological cross-reaction, but differed slightly in amino acid composition. If the age of the microbial isolated is similar to that of the salt deposits, they can be considered repositories of molecular information of great evolutionary interest.

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Helga Stan-Lotter
    • 1
    • 3
  • Michael Sulzner
    • 1
  • Eva Egelseer
    • 1
  • Cynthia F. Norton
    • 2
  • Lawrence I. Hochstein
    • 3
  1. 1.Institute of Microbiology and GeneticsUniversity of ViennaViennaAustria
  2. 2.Department of BiologyUniversity of Maine at AugustaMaineUSA
  3. 3.NASA Ames Research Center, Moffett FieldUSA

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