Current Microbiology

, Volume 8, Issue 4, pp 231–238

Description ofCampylobacter laridis, a new species comprising the nalidixic acid resistant thermophilicCampylobacter (NARTC) group

  • J. Benjamin
  • S. Leaper
  • R. J. Owen
  • M. B. Skirrow


Ten strains of the nalidixic acid-resistant thermophilicCampylobacter (NARTC) group, of which 2 were isolated from human feces, were compared with 12 reference strains representing various species ofCampylobacter. The NARTC strains were a homogeneous group with respect to their cell morphology and 28 physiological and biochemical characters. All were microaerophilic, motile (amphitrichate), gram-negative, curved, S-shaped or helical rods, and representative strains had mean DNA base compositions of 31 to 32 mol % G+C. Distinctive features of the 10 strains were resistance to nalidixic acid and anaerobic growth in the presence of trimethylamine N-oxide hydrochloride (TMAO). The latter feature may account for the common occurrence of NARTC strains in the fecal contents of seagulls. DNA-DNA hybridizations indicated high (≥76%) base sequence relatedness within the group and low (≤15%) relatedness to other species ofCampylobacter. The 10 strains were classified in the genusCampylobacter but they could not be assigned to any previously defined species. Therefore, a new species, with the nameCampylobacter laridis, is proposed for these 10 strains; the type strain is NCTC 11352.


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Literature Cited

  1. 1.
    Belland, R. J., Trust, T. J. 1982. Deoxyribonucleic acid sequence relatedness between thermophilic members of the genusCampylobacter. Journal of General Microbiology128:2515–2522.Google Scholar
  2. 2.
    Blaser, M. J., Moss, C. W., Weaver, R. E. 1980. Cellular fatty acid composition ofCampylobacter fetus. Journal of Clinical Microbiology11:448–451.Google Scholar
  3. 3.
    Burman, L. G. 1977. Apparent absence of transferable resistance to nalidixic acid in pathogenic gram-negative bacteria. Journal of Antimicrobial Chemotherapy3:509–516.Google Scholar
  4. 4.
    Cook, G. T. 1950. A plate test for nitrate reduction. Journal of Clinical Pathology3:359.Google Scholar
  5. 5.
    Cowan, S. T. 1974. Cowan and Steel's manual for the identification of medical bacteria. Cambridge: University Press.Google Scholar
  6. 6.
    Curtis, M. A. 1982. Cellular fatty acid profiles of campylobacters, pp. 234–241. In: D. G. Newell (ed.)Campylobacter, epidemiology, pathogenesis and biochemistry. Lancaster: MTP Press Ltd.Google Scholar
  7. 7.
    Garrod, L. P., Lambert, H. P., O'Grady, F. 1981. Antibiotics and chemotherapy, 5th ed. Edinburgh: Churchill Livingstone.Google Scholar
  8. 8.
    Hugh, R., Leifson, E. 1953. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. Journal of Bacteriology66:24–26.Google Scholar
  9. 9.
    Karmali, M. A., Allen, A. K., Fleming, P. C. 1981. Differentiation of catalase-positive campylobacters with special reference to morphology. International Journal of Systematic Bacteriology31:64–71.Google Scholar
  10. 10.
    King, E. O. 1957. Human infections withVibrio fetus and a closely related vibrio. Journal of Infectious Diseases101:119–128.Google Scholar
  11. 11.
    Leaper, S., Owen, R. J. 1981. Identification of catalaseproducingCampylobacter species based on biochemical characteristics and on cellular fatty acid composition. Current Microbiology31:31–35.Google Scholar
  12. 12.
    Leaper, S., Owen, R. J. 1982. Differentiation betweenCampylobacter jejuni and allied campylobacters by hybridization of deoxyribonucleic acids. FEMS Microbiology Letters15:203–208.Google Scholar
  13. 13.
    Luechtefeld, N. W., Wang, W. L. L. 1982. Hippurate hydrolysis by and triphenyltetrazolium tolerance ofCampylobacter fetus. Journal of Clinical Microbiology15:137–140.Google Scholar
  14. 14.
    Owen, R. J., Leaper, S. 1981. Base composition, size and nucleotide sequence similarities of genome deoxyribonucleic acids from species of the genusCampylobacter. FEMS Microbiology Letters12:395–400.Google Scholar
  15. 15.
    Owen, R. J., Legros, R. M., Lapage, S. P. 1978. Base composition, size and sequence similarities of genome deoxyribonucleic acids from clinical isolates ofPseudomonas putrefaciens. Journal of General Microbiology104:127–138.Google Scholar
  16. 16.
    Razi, R. H. H., Park, R. W. A., Skirrow, M. B. 1981. Two new tests for differentiating between strains ofCampylobacter. Journal of Applied Bacteriology50:55–57.Google Scholar
  17. 17.
    Skerman, V. B. D., McGowan, V., Sneath, P. H. A. (ed.). 1980. Approved lists of bacterial names. International Journal of Systematic Bacteriology30:225–420.Google Scholar
  18. 18.
    Skirrow, M. B., Benjamin, J. 1980. Differentiation of enteropathogenicCampylobacter. Journal of Clinical Pathology33:1122.Google Scholar
  19. 19.
    Skirrow, M. B., Benjamin, J. 1980. “1001” campylobacters: cultural characteristics of intestinal campylobacters from man and animals. Journal of Hygiene (Cambridge)85:427–442.Google Scholar
  20. 20.
    Tanner, A. C. R., Badger, S., Lai, C.-H., Listgarten, M. A., Visconti, R. A., Socransky S. S. 1981.Wolinella gen. nov.,Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and description ofBacteriodes gracilis sp. nov.,Wolinella recta sp. nov.,Campylobacter concisus sp. nov., andEikenella corrodens from humans with periodontal disease International Journal of Systematic Bacteriology31:432–445.Google Scholar
  21. 21.
    Taylor, D. E., De Grandis, S. A., Karmali, M. A., Fleming, P. C. 1982. Transmissible resistance inCampylobacter jejuni, pp. 207–210. In: D. G. Newell (ed.),Campylobacter, epidemiology, pathogenesis and biochemistry. Lancaster: MTP Press Ltd.Google Scholar
  22. 22.
    Véron, M., Chtelain R. 1973. Taxonomic study of the genusCampylobacter Sebald and Véron and designation of the neotype strain for the type speciesCampylobacter fetus (Smith and Taylor) Sebald and Véron. International Journal of Systematic Bacteriology23:122–134.Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • J. Benjamin
    • 1
  • S. Leaper
    • 2
  • R. J. Owen
    • 2
  • M. B. Skirrow
    • 1
  1. 1.Worcester Royal InfirmaryWorcesterUK
  2. 2.Central Public Health LaboratoryNational Collection of Type CulturesLondonUK

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