Journal of Industrial Microbiology

, Volume 8, Issue 2, pp 113–119

Mutants of a lovastatin-hyperproducingAspergillus terreus deficient in the production of sulochrin

  • V. A. Vinci
  • T. D. Hoerner
  • A. D. Coffman
  • T. G. Schimmel
  • R. L. Dabora
  • A. C. Kirpekar
  • C. L. Ruby
  • R. W. Stieber
Original Papers

DOI: 10.1007/BF01578762

Cite this article as:
Vinci, V.A., Hoerner, T.D., Coffman, A.D. et al. Journal of Industrial Microbiology (1991) 8: 113. doi:10.1007/BF01578762

Summary

A lovastatin-hyperproducing culture ofAspergillus terreus was shown to produce several co-metabolites extracted from whole broth. The predominant co-metabolite was the benzophenone, sulochrin, reported to arise from a polyketide biosynthetic pathway. This compound was targeted for elimination by classical mutagenesis and screening. A surface culture method employing microtiter, plates was used to ferment mutants for the primary screen. Qualitative determinations of lovastatin and sulochrin production were achieved by high-performance thin-layer chromatography. A mutant, strain AH6, which produced lovastatin titers equivalent to the parent culture and no detectable sulochrin was isolated. In addition, a lovastatin-hyperproducing mutant designated CB4 was capable of producing 16% more lovastatin and 30% less sulochrin than the parent culture in shake flask fermentations. In a pilot-scale 250-gallon fermentation, strain CB4 gave a 20% increase in lovastatin titer while producing 83% less sulochrin than the parent culture.

Key words

Microtiter fermentation Lovastatin Polyketide Strain improvement Screening 

Copyright information

© Society for Industrial Microbiology 1991

Authors and Affiliations

  • V. A. Vinci
    • 1
  • T. D. Hoerner
    • 1
  • A. D. Coffman
    • 1
  • T. G. Schimmel
    • 1
  • R. L. Dabora
    • 1
  • A. C. Kirpekar
    • 1
  • C. L. Ruby
    • 2
  • R. W. Stieber
    • 1
  1. 1.MCMDMerck & Co.ElktonUSA
  2. 2.MSDRLMerck & CoRahwayUSA

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