Current Microbiology

, Volume 8, Issue 5, pp 301–306 | Cite as

Tryptophan catabolism to indolepyruvic and indoleacetic acids byRhizobium japonicum L-259 mutants

  • Tsuneo Kaneshiro
  • Morey E. Slodki
  • Ronald D. Plattner
Article

Abstract

Tan-colored (tan) mutants, which retain the capacity to reduce acetylene, were selected and isolated fromRhizobium japonicum L-259 after growth in a glutamate-limited medium supplemented with tryptophan (trp). The mutants catabolized trp (500 mg per liter) to produce organe-colored fermentation broths containing extracellular indolepyruvic (IPA) and indoleacetic (IAA) acids. In contrast, parental strain L-259 produced a colorless broth containing only IAA when grown on the trp-supplemented medium. Trp alone was determined to be an incomplete nitrogen source for mutant growth and did not stimulate acetylenereduction activity.

Keywords

Nitrogen Fermentation Tryptophan Acetylene Nitrogen Source 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Literature Cited

  1. 1.
    Aragozzini, F., Ferrari, A., Pacini, N., Gualandris, R. 1979. Indole 3-lactic acid as a tryptophan metabolite produced byBifidobacterium spp. Applied and Environmental Microbiology38:544–546.Google Scholar
  2. 2.
    Badenoch-Jones, J., Summons, R. E., Entsch, B., Rolfe, B. G., Parker, C. W., Letham, D. S. 1982. Mass spectrometric identification of indole compounds produced byRhizobium strains. Biomedical Mass Spectrometry9:429–437.Google Scholar
  3. 3.
    Bender, R. A., Magasanik, B. 1977. Regulatory mutations in theKlebsiella aerogenes structural gene for glutamine synthetase. Journal of Bacteriology132:100–105.Google Scholar
  4. 4.
    Butts, W. C. 1972. Two-column gas chromatography of trimethylsilyl derivatives of biochemically significant compounds. Analytical Biochemistry46:187–199.Google Scholar
  5. 5.
    Garcia-Rodriguez, T., Guiterrez-Navarro, A. M., Garcia, R., Perez Silva, J. 1982. Indole acetic acid production byRhizobium: Effect of 2-ketoglutaric acid. Soil Biology and Biochemistry14:153–155.Google Scholar
  6. 6.
    Gartner, T. K., Riley, M. 1965. Isolation of mutants affecting tryptophanase production inEscherichia coli. Journal of Bacteriology89:313–318.Google Scholar
  7. 7.
    Goldberg, R. B., Bloom, F. R., Magasanik, B. 1976. Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria. Journal of Bacteriology127:114–119.Google Scholar
  8. 8.
    Gunsalus, I. C., Galeener, C. C., Stamer, J. R. 1955. Tryptophan cleavage. Tryptophanase fromE. coli. pp. 238–242. In: Colowick, S. P., Kaplan, N. O. (eds.), Methods in Enzymology, vol. 2. New York: Academic Press.Google Scholar
  9. 9.
    Hartmann, T., Glombitza, K.-W. 1967. Der Tryptophanabbau beiRhizobium leguminosarum. Archiv für Mikrobiologie56:1–8.Google Scholar
  10. 10.
    Hayaishi, O., Stanier, R. Y. 1951. The bacterial oxidation of tryptophan. III. Enzymatic activities of cell-free extracts from bacteria employing the aromatic pathway. Journal of Bacteriology62:691–709.Google Scholar
  11. 11.
    Hillman, J. R., Math, V. B., Medlow, G. C. 1977. Apical dominance and the levels of indole acetic acid inPhaseolus lateral buds. Planta134:191–193.Google Scholar
  12. 12.
    Kaneshiro, T., Kurtzman, M. A. 1982. Glutamate as a differential nitrogen source for the characterization of acetylene-reducingRhizobium strains. Journal of Applied Bacteriology52:201–207.Google Scholar
  13. 13.
    Kaneshiro, T., Newton, J. W., Selke, E., Slodki, M. E. 1980. Dinitrogen (15N2) fixation and acetylene reduction in freeliving strains ofRhizobium. Current Microbiology3:279–281.Google Scholar
  14. 14.
    Kaper, J. M., Veldstra, H. 1958. On the metabolism of tryptophan byAgrobacterium tumefaciens. Biochimica et Biophysica Acta30:401–420.Google Scholar
  15. 15.
    Kefford, N. P., Brockwell, J., Zwar, J. A. 1960. The symbiotic synthesis of auxin by legumes and nodule bacteria and its role in nodule development. Australian Journal of Biological Science13:456–467.Google Scholar
  16. 16.
    Ludwig, R. A., Signer, E. R. 1977. Glutamine synthetase and control of nitrogen fixation inRhizobium. Nature (London)267:245–248.Google Scholar
  17. 17.
    Magasanik, B., Prival, M. J., Brenchley, J. E., Tyler, B. M., DeLeo, A. B., Streicher, S. L., Bender, R. A., Paris, C. G. 1974. Glutamine synthetase as a regulator of enzyme synthesis, pp. 119–138. In: Horecker, B. L., Stadtman, E. R. (eds.). Current Topics in Cellular Regulation, vol. 8. New York: Academic Press.Google Scholar
  18. 18.
    Miller, I. L., Adelberg, E. A. 1953. The mechanism of kynureninase action. Journal of Biological Chemistry205:691–698.Google Scholar
  19. 19.
    Ng, H., Gartner, T. K. 1963. Selection of mutants ofEscherichia coli constitutive for tryptophanase. Journal of Bacteriology85:245–246.Google Scholar
  20. 20.
    Paris, C. G., Magasanik, B. 1981. Tryptophan metabolism inKlebsiella aerogenes: Regulation of the utilization of aromatic amino acids as sources of nitrogen. Journal of Bacteriology145:257–265.Google Scholar
  21. 21.
    Prival, M. J., Brenchley, J. E., Magasanik, B. 1973. Glutamine synthetase and the regulation of histidase formation inKlebsiella aerogenes. Journal of Biological Chemistry248:4334–4344.Google Scholar
  22. 22.
    Rao, V. R., Darrow, R. A., Keister, D. L. 1978. Effect of oxygen tension on nitrogenase and on glutamine synthetases I and II inRhizobium japonicum 61A76. Biochemical and Biophysical Research Communications81:224–231.Google Scholar
  23. 23.
    Rigaud, J. 1970. L'acide indolyl 3-lactique et son metabolisme chezRhizobium. Archiv für Mikrobiologie72:297–307.Google Scholar
  24. 24.
    Saric, Z., Milic, V., Hazem, T. 1978. Synthesis of indole, gibberellin, and phenol plant growth substances by someRhizobium japonicum strains of various efficiency. Arhiv za Poljoprivredne Nauke 31:29–41 (Serbo-Croatian). In: Chemical Abstracts90:117648s.Google Scholar
  25. 25.
    Smith, O. H., Yanofsky, C. 1962. Enzymes involved in biosynthesis of tryptophan. III. Tryptophan synthetase, pp. 801–806. In: Colowick, S. P., Kaplan, N. O. (eds.), Methods in Enzymology, vol. 5. New York: Academic Press.Google Scholar
  26. 26.
    Stadtman, E. R., Ginsburg, A. 1974. The glutamine synthetase ofEscherichia coli: Structure and control, pp. 755–807. In: Boyer, P. D. (ed.). The Enzymes, vol. 10. New York: Academic Press.Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Tsuneo Kaneshiro
    • 1
  • Morey E. Slodki
    • 1
  • Ronald D. Plattner
    • 1
  1. 1.Northern Regional Research Center, Agricultural Research ServiceU.S. Department of AgriculturePeoria

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