Advertisement

Current Microbiology

, Volume 24, Issue 5, pp 275–280 | Cite as

Inorganic phosphate transport inAcinetobacter lwoffi

  • Jacob Yashphe
  • Hemant Chikarmane
  • Maria Iranzo
  • Harlyn O. Halvorson
Article

Abstract

The transport of inorganic phosphate has been studied inAcinetobacter lwoffi JW11. During growth on excess phosphate, only one transport system was present, with an apparent Km of 1.4 μM. When cells were starved for phosphate, a second uptake system with an apparent Km of 110 nM was also synthesized. The two transport systems could be distinguished by differing sensitivities to the phosphate analogs arsenate and 2-aminoethylphosphonate. Both systems were inhibited by carbonylcyanidem-chlorophenylhydrazone, and to a lesser extent by Na azide. The high-affinity transport system was inactivated by osmotic shock treatment and by spheroplast formation. Preliminary evidence for a phosphate-binding protein in the osmotic shock fluid is presented. The isolation of a mutant constitutive for the high-affinity transport system is described.

Keywords

Phosphate Arsenate Azide Transport System Preliminary Evidence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Bennett RL, Malamy MH (1970) Arsenate-resistant mutants ofEscherichia coli and phosphate transport. Biochem Biophys Res Commun 40:496–503Google Scholar
  2. 2.
    Berger EA, Hippel LA (1974) Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases inE. coli. J Biol Chem 249:7747–7755Google Scholar
  3. 3.
    Blasco F, Ducet G, Azoulay E (1976) Mise en évidence de deux systèmes de transport du phosphate chez Candida tropicalis. Biochimie 58:351–357Google Scholar
  4. 4.
    Burns DJW, Beever RE (1977) Kinetic characterization of the two phosphate uptake systems in the fungusNeurospora crassa. J Bacteriol 132:511–519Google Scholar
  5. 5.
    Deane EM, O'Brien RW (1981) Uptake of phosphate by symbiotic and free-living dinoflagellates. Arch Microbiol 128:307–310Google Scholar
  6. 6.
    Friedberg I (1977) Phosphate transport inMicrococcus lysodeikticus. Biochim Biophys Acta 466:451–460Google Scholar
  7. 7.
    Halvorson HO, Suresh N, Roberts MF, Coccia M, Chikarmane H (1987) Metabolically active surface polyphosphate pool inAcinetobacter lwoffi. In: Torriani-Gorini A, Rothman FG, Silver S, Wright A, Yagil E (eds) Phosphate metabolism and cellular regulation in microorganisms. Washington: American Society for Microbiology, pp 220–224Google Scholar
  8. 8.
    Harold FM, Baarda JM (1966) Interaction of arsenate with phosphate-transport systems in wild-type and mutantStreptococcus faecalis. J Bacteriol 91:2257–2262Google Scholar
  9. 9.
    Krueger RD, Harper SH, Campbell JW, Fahrney DE (1986) Kinetics of phosphate uptake, growth, and accumulation of cyclic diphosphoglycerate in a phosphate-limited continuous culture ofMethanobacterium thermoautotrophicum. J Bacteriol 167:49–56Google Scholar
  10. 10.
    Lacoste A-M, Cassaigne A, Neuzil E (1981) Transport of inorganic phosphate inPseudomonas aeruginosa. Curr Microbiol 6:115–120Google Scholar
  11. 11.
    Makino K, Shinagawa H, Amemura M, Nakata A (1986) Nucleotide sequence of thephoB gene, the positive regulatory gene for the phosphate regulon ofEscherichia coli K-12. J Mol Biol 190:37–44Google Scholar
  12. 12.
    Medveczky N, Rosenberg H (1971) Phosphate transport inEscherichia coli. Biochim Biophys Acta 241:494–506Google Scholar
  13. 13.
    Neu HC, Chou J (1967) Release of surface enzymes inEnterobacteriaceae by osmotic shock. J Bacteriol 94:1934–1945Google Scholar
  14. 14.
    Pardee AB (1966) Purification and properties of a sulfate-binding protein fromSalmonella typhimurium. J Biol Chem 241:5886–5892Google Scholar
  15. 15.
    Pool K, Hancock REW (1984) Phosphate transport inPseudomonas aeruginosa: involvement of a periplasmic phosphate-binding protein. Eur J Biochem 144:607–612Google Scholar
  16. 16.
    Rosenberg H (1987) Phosphate transport in prokaryotes. In: Rosen BP, Silver S (eds) Ion transport in prokaryotes. New York: Academic Press, pp 205–248.Google Scholar
  17. 17.
    Rosenberg H, Medveczky N, La Nauze JM (1969) Phosphate transport inBacillus cereus. Biochim Biophys Acta 193:159–167Google Scholar
  18. 18.
    Rosenberg H, Gerdes RG, Chegwidden K (1977) Two systems for the transport of phosphate inEscherichia coli. J Bacteriol 131:505–511Google Scholar
  19. 19.
    Russell LM, Rosenberg H (1980) The nature of the link between potassium transport and phosphate transport inE. coli. Biochem J 188:715–723Google Scholar
  20. 20.
    Smart JB, Dilworth MJ, Robson AD (1984) Effect of phosphorus supply on phosphate uptake and alkaline phosphatase activity in Rhizobia. Arch Microbiol 140:281–286Google Scholar
  21. 21.
    Suresh N, Warburg R, Timmerman M, Wells J, Coccia M, Roberts MF, Halvorson HO (1985) New strategies for the isolation of microorganisms responsible for phosphate accumulation. Water Sci Technol 17:99–111Google Scholar
  22. 22.
    Surin BP, Rosenberg H, Cox GB (1985) Phosphate-specific transport system ofEscherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol 161:189–198Google Scholar
  23. 23.
    Torriani A, Ludtke D (1985) The Pho regulon ofEscherichia coli K-12. In: Schaechter M, Neidhardt FC, Ingraham J, Kjeldgaard NO (eds) Molecular biology of bacterial growth. Boston: Jones and Barlett, pp 224–242Google Scholar
  24. 24.
    Weiden PL, Epstein W, Schultz SG (1967) Cation transport inE. coli. VII. Potassium requirement for phosphate uptake. J Gen Physiol 50:1641–1661Google Scholar
  25. 25.
    Willsky GR, Malamy MH (1980a) Characterization of two genetically separable inorganic phosphate transport systems inEscherichia coli. J Bacteriol 144:356–365Google Scholar
  26. 26.
    Willsky GR, Malamy MH (1980b) Effect of arsenate on inorganic phosphate transport inEscherichia coli. J Bacteriol 144:366–374Google Scholar
  27. 27.
    Yashphe J, Chikarmane H, Iranzo M, and Halvorson HO (1990) Phosphatases ofAcinetobacter lwoffi. Localization, and regulation of synthesis by orthophosphate. Curr Microbiol 20:273–280Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1992

Authors and Affiliations

  • Jacob Yashphe
    • 1
  • Hemant Chikarmane
    • 1
  • Maria Iranzo
    • 1
  • Harlyn O. Halvorson
    • 1
  1. 1.Marine Biological LaboratoryWoods HoleUSA

Personalised recommendations