Current Microbiology

, Volume 15, Issue 1, pp 1–4 | Cite as

Medium and temperature dependence of decarboxylase reactions inAeromonas spp.

  • Martin Altwegg
  • Alexander von Graevenitz
  • Jaqueline Zollinger-Iten
Article

Abstract

Decarboxylase/dihydrolase activities inAeromonas spp. are important as diagnostic tools and indicators of enterotoxin production. We have analyzed the following media at 25°C, 29°C, and 37°C, respectively, for their ability to detect such activities: Møller's, Falkow's, and Fay and Barry's (F&B) containing ornithine, lysine, and arginine, respectively, as well as motility-indole-ornithine (MIO) medium and lysine decarboxylase broth with 0.1% agar (LDC). In order to retain ornithine negativity, but to get as much positivity as possible for arginine, optimal incubation conditions were 29°C for 96 h (Møller), 48 h (Falkow, MIO, and LDC), and 24 h (F&B). The F&B medium proved to be the most sensitive for the detection of lysine decarboxylase, a positive test being highly correlated with the two speciesA. hydrophila andA. sobria, and we suggest its use for routine detection of decarboxylase/dihydrolase activities.

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • Martin Altwegg
    • 1
  • Alexander von Graevenitz
    • 1
  • Jaqueline Zollinger-Iten
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of ZurichZurichSwitzerland

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