, Volume 3, Issue 1, pp 381–391 | Cite as

The antibody response during amphibian ontogeny

  • L. Du Pasquier
  • J. Haimovich


The maturation of the anti DNP-response in adult and larval amphibians has been compared in two species of anurans,Rana catesbeiana andXenopus laevis. IgM responses were very similar in larvae and adults of both species. Specific IgG antibody synthesis, studied inRana catesbeiana only, was not obvious in larval primary response but was easily detectable in adults. Although metamorphosis results in profound changes in the lymphoid system associated with the apparent acquisition of self tolerance to new antigens, immunological memory of antigens injected during larval life ofRana andXenopus persists after the metamorphosis period.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baculi, B.S. and Cooper, E.L.: Lymphomyeloid organs of Amphibia. IV. Normal histology in larval and adultRana catesbeiana.J. Morphol. 126:467–476, 1968Google Scholar
  2. Bernardini, N., Chardonnens, X., and Simon, D.: Développement après la métamorphose de compétences immunologiques envers les homogreffes cutanées chezXenopus laevis Daudin.C.R. Acad. Sci. (Paris) 209:1011–1014, 1969Google Scholar
  3. Chardonnens, X. and Du Pasquier, L.: Induction of skin allograft tolerance during metamorphosis of the toadXenopus laevis: a possible model of studying the generation of self tolerance to histocompatibility antigens.Eur. J. Immunol. 5:569–573, 1973Google Scholar
  4. Clem, L.W. and Small, P.A.: Phylogeny of immunoglobulin structure and function. V. Valences and association constants of teleost antibodies to a haptenic determinant.J. Exp. Med. 155:385–400, 1970Google Scholar
  5. Decker, J. and Sercarz, E.: Antigen binding cells and the generation of diversity.Amer. Zool. 15:189–197, 1975Google Scholar
  6. Du Pasquier, L.: Ontogeny of the immune response in animals having less than one million lymphocytes: the larvae of the toadAlytes obstetricans.Immunology 19:353–362, 1970Google Scholar
  7. Du Pasquier, L.: Ontogeny of the immune response in cold-blooded vertebrates.Curr. Top. Microbiol. Immunol. 61:37–88, 1973Google Scholar
  8. Du Pasquier, L. and Chardonnens, X.: Genetic aspect of the tolerance to allografts induced at metamorphosis in the toadXenopus laevis.Immunogenetics 2:431–440, 1975Google Scholar
  9. Du Pasquier, L. and Haimovich, J.: Changes in affinity of IgM antibodies in amphibian larvae.Eur. J. Immunol. 4:580–583, 1974Google Scholar
  10. Du Pasquier, L., Weiss, L.: The thymus during the ontogeny of the toadXenopus laevis: growth, membrane bound immunoglobulins and mixed lymphocyte reaction.Eur. J. Immunol. 5:773–777, 1973Google Scholar
  11. Elkan, E.: Amphibians.In: A.N. Worden and W. Lane-Petter (eds.):UFAW Handbook of the Care and Managment of Laboratory Animals (2nd Ed.), pp. 804–815. Livingstone, Edinburgh, 1957Google Scholar
  12. Emmrich, F., Richter, R.F., and Ambrosius, H.: Immunoglobulin determinants on the surface of lymphoid cells of carps.Eur. J. Immunol. 5:76–78, 1975Google Scholar
  13. Gecsy, C.L., Green, P.C., and Steiner, L.A.: Immunoglobulins in the developing amphibianRana catesbeiana.J. Immunol. 111:1261–1261, 1913Google Scholar
  14. Goidl, E.A. and Siskind, G.W.: Ontogeny of B lymphocyte function. I. Restricted heterogeneity of the antibody response of B lymphocytes from neonatal and fetal mice.J. Exp. Med. 140:1285–1302, 1914Google Scholar
  15. Hadji-Azimi, I.: Studies onXenopus laevis immunoglobulins.Immunology 21:463–474, 1971Google Scholar
  16. Haimovich, J. and Du Pasquier, L.: Specificity of antibodies in amphibian larvae possessing a small number of lymphocytes.Proc. Nat. Acad. Sci. USA.70:1898–1902, 1973Google Scholar
  17. Haimovich, J., Novik, N., and Sela, M.: Inhibition of the inactivation of modified phage.Isr. J. Med. Sci. 5:438, 1969Google Scholar
  18. Marchalonis, J.J. and Atwell, J.L.: Phylogenetic emergence of distinct immunogloublin classes.In L'Etude phylogénique et ontogénique de la response immunitaire et son apport à la théorie immunologique, pp. 153–162. Inserm., Paris, 1973Google Scholar
  19. Moticka, E.J., Brown, B.A., and Cooper, E.L.: Immunoglobulin synthesis in bullfrog larvae.J. Immunol. 110:855–861, 1973Google Scholar
  20. Nieuwkoop, P.D. and Faber, J.:Normal Table of Xenopus laevis (Daudin). North Holland, Amsterdam, 1967Google Scholar
  21. Rosenquist, G.L. and Hoffman, R.Z.: The production of anti-DNP antibody in the bullfrogRana catesbeiana.J. Immunol. 105:1499–1505, 1972Google Scholar
  22. Segal, S., Globerson, A., Feldman, M., Haimovich, J., and Sela, M.:In vitro induction of a primary response to the dinitrophenyl determinant.J. Exp. Med. 132:93–99, 1970Google Scholar
  23. Siskind, G.W. and Benacerraf, B.: Cell selection by antigen in the immune response.Adv. Immunol. 10:1–50, 1969Google Scholar
  24. Spear, P.G., Wang, A.L., Rutishauser, U., and Edelman, G.M.: Characterization of splenic lymphoid cells in fetal and newborn mice.J. Exp. Med. 155:557–573, 1973Google Scholar
  25. Urbain, J., Van Acker, A., de Vos-Cloetens, C.H., and Urbain-Vansanten, G.: Increase and decrease in binding affinity of antibodies during the immune response.Immunochemistry 9:121–136, 1972Google Scholar
  26. Volpe, E.P.: Transplantation immunity and tolerance in Anurans.Transplant. Proc. 2:286–292, 1970Google Scholar
  27. Witschi, E.:Development of Vertebrates, pp. 78–92, Saunders, Philadelphia, 1956Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1976

Authors and Affiliations

  • L. Du Pasquier
    • 1
  • J. Haimovich
    • 2
  1. 1.Basel Institute for ImmunologyBasel 5Switzerland
  2. 2.The Weizmann Institute of ScienceRehovotIsrael

Personalised recommendations