Current Microbiology

, Volume 27, Issue 1, pp 47–50 | Cite as

Chemotaxis towards sugars inChlamydomonas reinhardtii

  • Elena V. Ermilova
  • Zhanna M. Zalutskaya
  • Boris V. Gromov


Chemotaxis ofChlamydomonas reinhardtii 137c cells towards maltose and sucrose was observed by capillary assay. The threshold concentration was approximately 10−5m for maltose and 10−3m for sucrose. The peak accumulation of cells occurred at 10−3m for maltose and 10−2m for sucrose. A selection procedure for chemotaxis mutants was developed. Mutant strain CHE-1 was not attracted by maltose; mutant strain CHE-2 was not attracted by sucrose. Addition of attractant fully inhibited photoaccumulation of cells. After a period of time the photoresponse of cells recovered. Under the conditions of our experiments the period of adaptation lasted 15–20 min in wild-type cells and 4–5 min in mutant cells on the given sugar. Glucose and acetate did not attract cells ofChlamydomonas. Added to the medium, these compounds had no effect on the photoaccumulation of cells.


Glucose Acetate Sugar Sucrose Maltose 
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Literature Cited

  1. 1.
    Boscov JS, Feinleib ME (1979) Phototactic response ofChlamydomonas to flashes of light. II. Response of individual cells. Photochem Photobiol 30:499–505Google Scholar
  2. 2.
    Devreotes PN, Steck TL (1979) Cyclic 3′, 5′ AMP relay inDictyostelium discoideum. II. Requirements for the initiation and termination of the responses. J Cell Biol 80:300–309Google Scholar
  3. 3.
    Diehn B, Feinleib M, Haupt W, Hildebrand E, Lenci F, Nultsch W (1977) Terminology of behavioral responses of motile microorganisms. Photochem Photobiol 26:559–560Google Scholar
  4. 4.
    Ekelund NGA, Dolle R, Nultsch W (1988) Phototactic responses ofChlamydomonas reinhardtii in electric field. Physiologia Plantarum 73:268–270Google Scholar
  5. 5.
    Koshland DE (1979) A model regulatory system: bacterial chemotaxis. Physiol Rev 59:811–862Google Scholar
  6. 6.
    Levine RP, Ebersold WT (1958) Gene recombination inChlamydomonas reinhardtii. Cold Spring Harbor Symp Quant Biol 23:395–410Google Scholar
  7. 7.
    Sjoblad RD, Fredrikse PH (1981) Chemotactic responses ofChlamydomonas reinhardtii. Mol Cell Biol 1:1057–1060Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1993

Authors and Affiliations

  • Elena V. Ermilova
    • 1
  • Zhanna M. Zalutskaya
    • 1
  • Boris V. Gromov
    • 1
  1. 1.Laboratory of MicrobiologyBiological Institute of St. Petersburg UniversitySt. PetersburgRussia

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