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Current Microbiology

, Volume 27, Issue 1, pp 47–50 | Cite as

Chemotaxis towards sugars inChlamydomonas reinhardtii

  • Elena V. Ermilova
  • Zhanna M. Zalutskaya
  • Boris V. Gromov
Article

Abstract

Chemotaxis ofChlamydomonas reinhardtii 137c cells towards maltose and sucrose was observed by capillary assay. The threshold concentration was approximately 10−5m for maltose and 10−3m for sucrose. The peak accumulation of cells occurred at 10−3m for maltose and 10−2m for sucrose. A selection procedure for chemotaxis mutants was developed. Mutant strain CHE-1 was not attracted by maltose; mutant strain CHE-2 was not attracted by sucrose. Addition of attractant fully inhibited photoaccumulation of cells. After a period of time the photoresponse of cells recovered. Under the conditions of our experiments the period of adaptation lasted 15–20 min in wild-type cells and 4–5 min in mutant cells on the given sugar. Glucose and acetate did not attract cells ofChlamydomonas. Added to the medium, these compounds had no effect on the photoaccumulation of cells.

Keywords

Glucose Acetate Sugar Sucrose Maltose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1993

Authors and Affiliations

  • Elena V. Ermilova
    • 1
  • Zhanna M. Zalutskaya
    • 1
  • Boris V. Gromov
    • 1
  1. 1.Laboratory of MicrobiologyBiological Institute of St. Petersburg UniversitySt. PetersburgRussia

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