Current Microbiology

, Volume 27, Issue 1, pp 5–9 | Cite as

Regular proteinaceous layers ofThermococcus stetteri cell envelope

  • Georgy M. Gongadze
  • Alla S. Kostyukova
  • Margarita L. Miroshnichenko
  • Elizaveta A. Bonch-Osmolovskaya


Proteinaceous layers of theThermococcus stetteri cell envelope were investigated and found to consist of regularly arrayed subunits 18 nm in diameter. According to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two major proteins were present. They were glycosylated and had molecular weights of 80,000 and 210,000. In addition to two external regular proteinaceous layers, cells ofT. stetteri were found to have internal regular layers tightly attached to the cytoplasmic membrane. In the region of flagella attachment to the cell, polar membrane-like structures were found in the cytoplasm.


Sodium Molecular Weight Electrophoresis Dodecyl Sodium Dodecyl 
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Copyright information

© Springer-Verlag New York Inc. 1993

Authors and Affiliations

  • Georgy M. Gongadze
    • 1
  • Alla S. Kostyukova
    • 2
  • Margarita L. Miroshnichenko
    • 3
  • Elizaveta A. Bonch-Osmolovskaya
    • 3
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchino Moscow regionRussia
  2. 2.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow regionRussia
  3. 3.Institute of MicrobiologyRussian Academy of SciencesMoscowRussia

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