Journal of Industrial Microbiology

, Volume 6, Issue 4, pp 253–261

Recycle of cellulases and the use of lignocellulosic residue for enzyme production after hydrolysis of steam-pretreated aspenwood

  • Charlene M. Hogan
  • Mary Mes-Hartree
Original Papers

DOI: 10.1007/BF01575870

Cite this article as:
Hogan, C.M. & Mes-Hartree, M. Journal of Industrial Microbiology (1990) 6: 253. doi:10.1007/BF01575870

Summary

Various modes of substrate and enzyme addition were used to hydrolyze a 10% concentration (w/v) of steam-exploded, water-and-alkali extracted aspenwood withTrichoderma harzianum E58 cellulases. Although cellulose conversion was high (94–100%), enzyme recovery was low in all cases. Low enzyme recovery was due to a combination of thermal inactivation and adsorption of the cellulases onto the lignocellulosic residue. Enzyme recycle was not feasible as the activity of the recovered cellulases towards crystalline cellulose was low. However, the residual material from enzyme hydrolysis was a suitable carbon source for cellulase enzyme production byT. harzianum based on enzyme yield and hydrolytic potential. These residues could only be used up to a 1% substrate concentration, since at higher substrate loadings cellulase production was reduced, likely because of lignin inhibitors.

Key words

Cellulase production Aspenwood, steam-exploded Trichoderma harzianum 

Copyright information

© Society for Industrial Microbiology 1990

Authors and Affiliations

  • Charlene M. Hogan
    • 1
  • Mary Mes-Hartree
    • 1
  1. 1.Department of Biotechnology and Wood ChemistryForintek Canada Corp.OttawaCanada

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