Current Microbiology

, Volume 10, Issue 2, pp 105–109

Characterization of the antigens involved in serotyping strains ofCampylobacter jejuni by passive hemagglutination

  • Dennis M. Jones
  • Andrew J. Fox
  • Joan Eldridge
Article

Abstract

Components of outer membrane preparations, heated saline extracts, and phenolwater lipopolysaccharide extracts obtained from strains ofCampylobacter jejuni representing seven passive hemagglutination serotypes (Penner serotypes 1–4, 13, 16, and 50) were electrophoresed in sodium dodecyl sulfate-polyacrylamide gels. Tests of gel eluates demonstrated that lipopolysaccharide antigens are involved in serotypingC. jejuni by passive hemagglutination and that other cell surface components have no activity. This finding was confirmed by hemagglutination inhibition. In the typing ofC. jejuni by passive hemagglutination, each serotype is probably defined by the presence of one or more specific lipopolysaccharides. These findings may lead to a clarification of the serotyping nomenclature for those systems that depend on passive hemagglutination. It is recommended that a single internationally agreed numbering system be adopted for lipopolysaccharides derived fromC. jejuni.

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Literature Cited

  1. 1.
    Abbott, J. D., Dale, B. A. S., Eldridge, J., Jones, D. M., Sutcliffe, E. M. 1980. Serotyping ofCampylobacter jejuni/coli. Journal of Clinical Pathology33:762–766.Google Scholar
  2. 2.
    Hebert, G. A., Hollis, D. G., Weaver, R. E., Steigerwalt, A. G., McKinney, R. M., Brenner, D. J. 1983. Serogroups ofCampylobacter jejuni, Campylobacter coli andCampylobacter fetus defined by direct immunofluorescence. Journal of Clinical Microbiology17:529–538.Google Scholar
  3. 3.
    Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227:680–685.Google Scholar
  4. 4.
    Lauwers, S., Vlaes, L., Butzler, J. P. 1981. Campylobacter serotyping and epidemiology. Lanceti:158–159.Google Scholar
  5. 5.
    Lior, H., Woodward, D. L., Edgar, J. A., Laroche, L. J., Gill, P. 1982. Serotyping ofCampylobacter jejuni by slide agglutination. Journal of Clinical Microbiology15:761–768.Google Scholar
  6. 6.
    Logan, S. M., Trust, T. J. 1982. Outer membrane characteristics ofCampylobacter jejuni. Infection and Immunity38:898–906.Google Scholar
  7. 7.
    O'Neill, G. J., Todd, J. P. 1961. Extraction of nucleic acid free lipopolysaccharide from gram-negative bacteria. Nature (London)190:344–345.Google Scholar
  8. 8.
    Penner, J. L., Hennessy, J. N. 1980. Passive hemagglutination technique for serotypingCampylobacter fetus subsp.jejuni on the basis of heat stable antigens. Journal of Clinical Microbiology12:732–737.Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Dennis M. Jones
    • 1
  • Andrew J. Fox
    • 1
  • Joan Eldridge
    • 1
  1. 1.Public Health LaboratoryWithington HospitalManchesterUK

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