Current Microbiology

, Volume 28, Issue 3, pp 139–143 | Cite as

Streptococcus shiloi andStreptococcus difficile: Two new streptococcal species causing a meningoencephalitis in fish

  • Avi Eldar
  • Yitzhak Bejerano
  • Herve Bercovier


A bacterial meningoencephalitis in St. Peter's fish (Tilapia spp.) and trout (Oncorhynchus mykiss) appeared in Israel in 1986 and rapidly spread throughout the country, causing considerable economic losses. We isolated and identified the agents of this disease. They were Gram-positive, nonsporulating, facultatively anaerobic chain-forming cocci, catalase negative. They were able to grow at pH 9.6 but not at 10°C nor at 45°C nor in the presence of 40% (vol/vol) bile salts or in the presence of 6.5% NaCl (wt/vol). DNA base composition (G+C%=37%), DNA-DNA hybridizations, biochemical and serological studies indicated that these strains constitute two new distinct species of streptococci that we propose to nameStreptococcus shiloi (type strain ND 2-16) andStreptococcus difficile (type strain ND 2-22). Unclassified isolates from Japan and from Taiwan belonged toStreptococcus shiloi orStreptococcus difficile, showing the cosmopolitan distribution of these two newly described species.


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Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • Avi Eldar
    • 1
  • Yitzhak Bejerano
    • 2
  • Herve Bercovier
    • 1
  1. 1.Department of Clinical MicrobiologyThe Hebrew University-Hadassah Medical SchoolJerusalemIsrael
  2. 2.The Nir-David Laboratory for Fish DiseasesNir-DavidIsrael

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