Ultrastructural study of two rumen fungi:Piromonas communis andSphaeromonas communis
- 39 Downloads
- 6 Citations
Abstract
Two species of strictly anaerobic rumen fungi,Sphaeromonas communis andPiromonas communis, were examined by light and electron microscopy (scanning and transmission). Although morphologically different (vegetative body, number of flagella per zoospore), the ultrastructure of these two microorganisms was rather similar to that ofNeocallimastix frontalis andN. patriciarum. Two types of organelles were regularly found, i.e., isolated or associated ribosomes in the form of aggregates and hydrogenosome-like organelles with an amorphous content that may be involved in energy generation for these mitochondria-free strictly anaerobic fungi. UnlikeN. frontalis, the distribution of organelles was homogenous.
Keywords
Microscopy Electron Microscopy Energy Generation Ultrastructural Study Amorphous ContentPreview
Unable to display preview. Download preview PDF.
Literature Cited
- 1.Fonty G, Breton A, Fevre M, Citron A, Hebraud M, Gouet Ph (1987) Isolement et caractérisation des champignons anaérobies stricts du rumen de moutons. Premiers résultats. Reprod Nutr Develop 27 (1B):237–238Google Scholar
- 2.Health IB, Bauchop T, Skipp A (1983) Assignmen of the rumen anaerobeNeocallimastix frontalis to the spizellomycetales (chytridiomycetes) on the basis of its polyflagellate zoospore ultrastructure. Can J Bot 61:295–307Google Scholar
- 3.Muller M (1980), pp. 127–142. In: Gooday GW, Lloyd D, Trinci APJ (eds) The eukaryotic microbial cell. Cambridge: Cambridge University PressGoogle Scholar
- 4.Munn EA, Orpin CG, Hall J (1981) Ultrastructural studies of the free zoospore of the rumen phycomyceteNeocallimastix frontalis. J Gen Microbiol 125:311–323PubMedGoogle Scholar
- 5.Orpin CG (1975) Studies on the rumen flagellateNeocallimastix frontalis. J Gen Microbiol 94:249–262Google Scholar
- 6.Orpin CG (1977) On the induction of zoosporogenesis in the rumen phycomycetesNeocallimastix frontalis, Piromonas communis andSphaeromonas communis. J Gen Microbiol 101:181–189PubMedGoogle Scholar
- 7.Orpin CG, Bountiff L (1978) Zoospore chemotaxis in the rumen phycomyceteNeocallimastix frontalis. J Gen Microbiol 104:113–122Google Scholar
- 8.Orpin CG, Munn EA (1986)Neocallimastix patriciarum sp nov a new member of theNeocallimasticaceae inhabiting the rumen of sheep. Trans Br Mycol Soc 86(1):178–181Google Scholar
- 9.Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212PubMedGoogle Scholar
- 10.Yarlett N, Orpin CG, Munn EA, Yarlett NC, Greenwood CA (1986) Hydrogenosomes in the rumen fungusNeocallimastix patriciarum. Biochem J 236:729–739PubMedGoogle Scholar