Somatic Cell and Molecular Genetics

, Volume 12, Issue 4, pp 415–420

Localization of multidrug resistance-associated DNA sequences to human chromosome 7

  • A. Fojo
  • R. Lebo
  • N. Shimizu
  • J. E. Chin
  • I. B. Roninson
  • G. T. Merlino
  • M. M. Gottesman
  • I. Pastan
Brief Communication

Abstract

Multidrug resistance in several human cell lines correlates with amplification or increased expression of two related DNA sequences, designated mdr1and mdr2.These DNA sequences were used as probes for hybridization with DNA with a panel of human-mouse somatic cell hybrids and from individual human chromosomes separated by fluorescence-activated chromosome sorting. By these assays, both mdr1and mdr2sequences were localized to chromosome 7.

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • A. Fojo
    • 1
  • R. Lebo
    • 2
  • N. Shimizu
    • 3
    • 4
  • J. E. Chin
    • 5
  • I. B. Roninson
    • 5
  • G. T. Merlino
    • 1
  • M. M. Gottesman
    • 1
  • I. Pastan
    • 1
  1. 1.Laboratory of Molecular BiologyNational Cancer Institute, National Institutes of HealthBethesda
  2. 2.Howard Hughes Medical Institute, Division of Genetics and Molecular Hematology, Department of MedicineUniversity of CaliforniaSan Francisco
  3. 3.Department of Molecular and Cellular BiologyUniversity of ArizonaTucson
  4. 4.Department of Molecular BiologyKeio University of MedicineTokyoJapan
  5. 5.University of Illinois College of Medicine at ChicagoChicago

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