Somatic Cell and Molecular Genetics

, Volume 12, Issue 4, pp 415–420

Localization of multidrug resistance-associated DNA sequences to human chromosome 7

  • A. Fojo
  • R. Lebo
  • N. Shimizu
  • J. E. Chin
  • I. B. Roninson
  • G. T. Merlino
  • M. M. Gottesman
  • I. Pastan
Brief Communication


Multidrug resistance in several human cell lines correlates with amplification or increased expression of two related DNA sequences, designated mdr1and mdr2.These DNA sequences were used as probes for hybridization with DNA with a panel of human-mouse somatic cell hybrids and from individual human chromosomes separated by fluorescence-activated chromosome sorting. By these assays, both mdr1and mdr2sequences were localized to chromosome 7.


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Literature cited

  1. 1.
    Akiyama, S.-I., Fojo, A.T., Hanover, J., Pastan, I., and Gottesman, M.M. (1985).Somat. Cell Mol. Genet. 11:117–126.PubMedGoogle Scholar
  2. 2.
    Beck, W.T. (1983).Cancer Treat. Rep. 67:875–882.PubMedGoogle Scholar
  3. 3.
    Beck, W.T., Mueller, R.J., and Tanzer, L.R. (1979).Cancer Res. 39:2070–2076.PubMedGoogle Scholar
  4. 4.
    Biedler, J.L., and Riehm, H. (1970).Cancer Res. 30:1174–1184.PubMedGoogle Scholar
  5. 5.
    Carlsen, S.A., Till, J.E., and Ling, V. (1976).Biochim. Biophys. Acta 455:900–912.PubMedGoogle Scholar
  6. 6.
    Ling, V., and Thompson, L.H. (1973).J. Cell. Physiol. 83:103–116.Google Scholar
  7. 7.
    Riehm, H., and Biedler, J.L. (1971).Cancer Res. 31:409–412.PubMedGoogle Scholar
  8. 8.
    See, Y.P., Carlsen, S.A., Till, J.E., and Ling, V. (1974).Biochim. Biophys. Acta 373:242–252.PubMedGoogle Scholar
  9. 9.
    Siegfried, J.A., Tritton, T.R., and Sartorelli, A.C. (1983).Eur. J. Cancer Clin. Oncol. 19:1133–1139.PubMedGoogle Scholar
  10. 10.
    Shen, D.-W., Cardarelli, C., Hwang, J., Cornwell, M., Richert, N., Ishii, S., Pastan, I., and Gottesman, M.M. (1986).J. Biol. Chem. 261:7762–7770.PubMedGoogle Scholar
  11. 11.
    Fojo, A.T., Akiyama, S.-I., Gottesman, M.M., and Pastan, I. (1985).Cancer Res. 45:3002–3007.PubMedGoogle Scholar
  12. 12.
    Richert, N., Akiyama, S.-I., Shen, D.-W., Gotteman, M.M., and Pastan, I. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:2330–2333.PubMedGoogle Scholar
  13. 13.
    Fojo, A.T., Whang-Peng, J., Gottesman, M.M., and Pastan, I. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:7661–7665.PubMedGoogle Scholar
  14. 14.
    Roninson, I., Chin, J., Choi, K., Gros, P., Housman, D., Fojo, A., Shen, D.-W., Gottesman, M.M., and Pastan, I. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:4538–4542.PubMedGoogle Scholar
  15. 15.
    Roninson, I.B., Abelson, H.T., Housman, D.E., Howell, N., and Varshavsky, A. (1984).Nature 309:626–628.PubMedGoogle Scholar
  16. 16.
    Gros, P., Croop, J., Roninson, I., Varshavsky, A., and Housman, D.E. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:337–341.PubMedGoogle Scholar
  17. 17.
    Shen, D.-W., Fojo, A., Chin, J.E., Roninson, I.B., Richert, N., Pastan, I., and Gottesman, M.M. (1986).Science 232:643–645.PubMedGoogle Scholar
  18. 18.
    Lebo, R.V., Gorin, F., Fletterick, R.J., Kao, F.-T., Cheung, M.-C., Bruce, B.D., and Kan, Y.W. (1984).Science 225:57–59.PubMedGoogle Scholar
  19. 19.
    Lebo, R.V., and Bastian, A.M. (1982).Cytometry 3:213–219.PubMedGoogle Scholar
  20. 20.
    Shimizu, N., Kondo, I., Gamou, S., Behzadian, M.A., and Shimizu, Y. (1984).Somat. Cell Mol. Genet. 10:45–53.PubMedGoogle Scholar
  21. 21.
    Behzadian, M.A., Shimizu, Y., Kondo, I., and Shimizu, N. (1982).Somat. Cell Genet. 8:347–362.PubMedGoogle Scholar
  22. 22.
    Fabricant, R.N., Delarco, J.E., and Todaro, G.S. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:565–569.PubMedGoogle Scholar
  23. 23.
    Eva, A., Robbins, K.C., Andersen, P.R., Srinivasan, A., Tronick, S.R., Reddy, E.P., Ellmore, N.W., Gorlen, A.T., Lautenberger, J.A., Papas, T.S., Westin, E.H., Wong-Staal, F., Gallo, R.C., and Aaronson, S.A. (1982).Nature 295:116–119.PubMedGoogle Scholar
  24. 24.
    Merlino, G.T., Ishii, S., Whang-Peng, J., Knutsen, T., Xu, Y.-H., Clark, A.J., Stratton, R.H., Wilson, R.K., Ma, D.P., Roe, B.A., Hunts, J.H., Shimizu, N., and Pastan, I. (1985).Mol. Cell. Biol. 5:1722–1734.PubMedGoogle Scholar
  25. 25.
    Schwartz, D.C., and Cantor, C.R. (1984).Cell 37:67–75.PubMedGoogle Scholar
  26. 26.
    Riordan, J.R., Deuchars, K., Kartner, H., Alon, H., Trent, J., and Ling, V. (1985).Nature 316:817–819.PubMedGoogle Scholar
  27. 27.
    Kartner, N., Evernden-Proelle, D., Bradley, G., and Ling, V. (1985).Nature 316:820–823.PubMedGoogle Scholar
  28. 28.
    Trent, J., Bell, D., Willard, H., and Ling, V. (1985).Hum. Gene Mapping 8 40:761.Google Scholar

Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • A. Fojo
    • 1
  • R. Lebo
    • 2
  • N. Shimizu
    • 3
    • 4
  • J. E. Chin
    • 5
  • I. B. Roninson
    • 5
  • G. T. Merlino
    • 1
  • M. M. Gottesman
    • 1
  • I. Pastan
    • 1
  1. 1.Laboratory of Molecular BiologyNational Cancer Institute, National Institutes of HealthBethesda
  2. 2.Howard Hughes Medical Institute, Division of Genetics and Molecular Hematology, Department of MedicineUniversity of CaliforniaSan Francisco
  3. 3.Department of Molecular and Cellular BiologyUniversity of ArizonaTucson
  4. 4.Department of Molecular BiologyKeio University of MedicineTokyoJapan
  5. 5.University of Illinois College of Medicine at ChicagoChicago

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