Abstract
The effect of glycerol on the growth, adhesion, and cellulolytic activity of two rumen cellulolytic bacterial species,Ruminococcus flavefaciens andFibrobacter succinogenes subsp.succinogenes, and of an anaerobic fungal species,Neocallimastix frontalis, was studied. At low concentrations (0.1–1%), glycerol had no effect on the growth, adhesion, and cellulolytic activity of the two bacterial species. However, at a concentration of 5%, it greatly inhibited their growth and cellulolytic activity. Glycerol did not affect the adhesion of bacteria to cellulose. The growth and cellulolytic activity ofN. frontalis were inhibited by glycerol, increasingly so at higher concentrations. At a concentration of 5%, glycerol totally inhibited the cellulolytic activity of the fungus. Thus, glycerol can be added to animal feed at low concentrations.
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Literature Cited
Akin DE (1979) Microscopic evaluation of forage digestion by rumen microorganisms—a review. J Animal Sci 48:701–710
Akin DE (1987) Association of rumen fungi with various forage grasses. Anim Feed Sci Technol 16:273–285
Bauchop T (1981) The anaerobic fungi in rumen fibre digestion. Agr Environ 6:339–348
Bergmann N, Starr DJ, Reulein S (1968) Glycerol metabolism and gluconeogenesis in the normal hypoglycemic ketotic sheep. Am J Physiol 215:874–880
Bradford DM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utlizing the principle of protein-dye binding. Annal Biochem 72:248–254
Cheng KJ, Stewart CS, Dinsdale D, Costerton JW (1983/1984) Electron microscopy of bacteria involved in the digestion of plant cell walls. Anim Feed Sci Technol 10:93–120
Cheng KJ, Kudo H, Duncan SH, Mesbah A, Stewart CS, Bernalier A, Fonty G, Costerton JW (1991) Prevention of fungal colonization and digestion of cellulose by the addition of methylcellulose. Can J Microbiol 37:484–487
Czerkawski JW, Breckenridge G (1972) Fermentation of various glycolytic intermediates and other compounds by rumen microorganisms with particular reference to methane production. Br J Nutr 27:131–146
Fonty G, Roger V, Andre C, Bonnemoy F, Gouet Ph (1991) Effect of glycerol on rumen cellulolytic bacteria and anaerobic fungi. Abstracts of the 21st Biennal Conference on Rumen Function, Chicago (November 12–14), p 38
Joblin KN (1981) Isolation, enumeration and maintenance of rumen anaerobic fungi in roll tubes. Appl Environ Microbiol 42:1119–1122
Latham MJ (1978) Quantitative aspects of the adhesion ofRuminococcus flavefaciens to plant cell walls. Proc Soc Gen Microbiol 5:108
Latham MJ, Hobbs DG, Harris PJ (1979) Adhesion of rumen bacteria to alkali-treated plant stems. Ann Rech Vet 10:244–245
Mittlbach M, Wörgetter M, Pernkopf J, Junek H (1983) Diesel fuel derived from vegetable oils: preparation and use of rape oil methylester. Energ Agric 2:369–384
Roger V, Fonty G, Komisarczuk-Bony S, Gouet Ph (1990) Effects of physico-chemical factors on the adhesion to cellulose Avicel of the ruminal bacteriaRuminococcus flavefaciens andFibrobacter succinogenes subsp.succinogenes. Appl Environ Microbiol 56:3081–3087
Scott HW, Dehority BA (1965) Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89:1169–1175
Thivend P, Fonty G, Jouany JP, Durand M, Gouet Ph (1985) Le fermenteur rumen. Reprod Nutr Dev 25:729–753
Trathnigg B, Mittelbach M (1990) Analysis of triglyceride methanolysis mixtures using isocratic H.P.L.C. with density detection. J Chromatogr 13:95–105
Wright DE (1969) Fermentation of glycerol by rumen microorganisms. NZ J Agric Res 12:281–286
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Roger, V., Fonty, G., Andre, C. et al. Effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi. Current Microbiology 25, 197–201 (1992). https://doi.org/10.1007/BF01570719
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DOI: https://doi.org/10.1007/BF01570719