Advertisement

Immunogenetics

, Volume 9, Issue 1, pp 261–272 | Cite as

Histocompatibility-2 system in wild mice

VIII. Frequencies of H-2 and Ia antigens in wild mice from Texas
  • W. R. Duncan
  • E. K. Wakeland
  • J. Klein
Original Investigations

Abstract

Ninety-six wild mice trapped at 13 localities in the state of Texas were tested in the dye-exclusion cytotoxic test with a battery of 49 oligospecific H-2 antisera. The antisera detected 36 class I (K and D) and 10 class II (Ia) antigens. The phenotypic frequencies of private class I antigens ranged from 1 to 20%, the majority of them being in the range between 1 and 5%. At least some of the higher frequencies resulted from the presence of more than one antibody in the typing reagents, and from other factors complicating the typing. We estimate that the frequencies of most of the class I alleles among Texas wild mice are 1% or less. This estimate leads to the prediction that at least 200 alleles exist in Texas mice at theH-2K locus, and another 200 alleles exist at theH-2D locus. Frequencies of most of the class I public antigens were in excess of 20%. In the sample of 96 mice, 46 different phenotypic combinations of private class I antigens were found, and the frequency of blanks (mice unreactive with any of the antibodies to private class I antigens) was 27%. The frequencies of private class II antigens ranged from 5 to 15%. Some of the public class II antigens, in particular those controlled by theE region, occurred with frequencies of 80% or higher. The class II antigens were found in 26 phenotypic combinations. No striking linkage disequilibrium was found either between K and D antigens, or between class I and class II antigens. The polymorphism of theK, A, andD region appears to be higher than that of the corresponding regions of the human or rat major histocompatibility complex. The polymorphism of theE region is significantly lower than that of theA, K, andD regions. The polymorphism of theA region is extensive.

Keywords

Linkage Disequilibrium Major Histocompatibility Complex Cytotoxic Test theE Region andD Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Albert, E. and Götze, D.: The major histocompatibility system in man.In D. Götze (ed.):The Major Histocompatibility System in Man and Animals; pp. 7–77, Springer-Verlag, New York, 1977Google Scholar
  2. Duncan, W. R., Wakeland, E. K., and Klein, J.: Heterozygosity ofH-2 loci in wild mice.Nature, in press, 1979Google Scholar
  3. Ivanyi, P., Démant, P., Vojtíšková, M., and Iványi, D.: Histocompatibility antigens in wild mice (Mus musculus).Transplant. Proc. 1:365–367, 1969PubMedGoogle Scholar
  4. Ivanyi, P. and Démant, P.: Preliminary studies on histocompatibility antigens in wild mice (Mus musculus).In W. Junk (ed.):Proc. 11th European Conf. on Animal Blood Groups and Biochem. Polymorphism; pp. 547–550, N. V. Publishers, The Hague, 1970Google Scholar
  5. Ivanyi, P. and Micková, M.: Testing of wild mice for “private” H-2 antigens. Cross reactions or nonspecific reactions?Transplantation 14:802–804, 1971Google Scholar
  6. Klein, J.: Histocompatibility-2 (H-2) polymorphism in wild mice.Science 168:1362–1364, 1970PubMedGoogle Scholar
  7. Klein, J.: Private and public antigens of the mouseH-2 system.Nature 229:635–637, 1971Google Scholar
  8. Klein, J.: Histocompatibility-2 system in wild mice. I. Identification of five newH-2 chromosomes.Transplantation 13:291–299, 1972PubMedGoogle Scholar
  9. Klein, J.: Polymorphism of theH-2 loci in wild mice.In R. H. Regamey and J. V. Sparck (eds.):International Symposium on HL-A Reagents; pp. 251–256, S. Karger, Basel, 1973Google Scholar
  10. Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Principles of Immunogenetics Applied to a Single System. Springer-Verlag, New York, 1975Google Scholar
  11. Klein, J.:H-2 mutations: Their genetics and effect on immune functions.Adv. Immunol. 26:55–146, 1978PubMedGoogle Scholar
  12. Klein, J.: The major histocompatibility complex of the mouse.Science 203:516–521, 1979PubMedGoogle Scholar
  13. Klein, J., Hauptfeld, V., and Hauptfeld, M.: Evidence for a fifth (G) region in theH-2 complex of the mouse.Immunogenetics 2:141–150, 1975Google Scholar
  14. Schwarz, E. and Schwarz, H. K.: The wild and commensal stocks of the house mouse,Mus musculus Linnaeus.J. Mammal. 24:59–72, 1943Google Scholar
  15. Shonnard, J. W., Cramer, D. V., Poloskey, P. E., Kunz, H. E., and Gill III, T. J.: Polymorphism of the major histocompatibility locus in the wild Norway rat.Immunogenetics 3:193–200, 1976Google Scholar
  16. Wakeland, E. K. and Klein, J.: Histocompatibility-2 system in wild mice. VII. Serological analysis of 29 wild-derivedH-2 haplotypes with antisera to inbred I-region antigens.Immunogenetics 8:27–39, 1979Google Scholar
  17. Zaleska-Rutczynska, Z. and Klein, J.: Histocompatibility-2 system in wild mice. V. Serological analysis of sixteen B10.W congenic lines.J. Immunol. 119:1903–1911, 1977PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1979

Authors and Affiliations

  • W. R. Duncan
    • 1
  • E. K. Wakeland
    • 2
  • J. Klein
    • 2
  1. 1.Department of Cell BiologyThe University of Texas Health Science CenterDallas
  2. 2.Abteilung ImmungenetikMax-Planck-lnstitut für BiologieTübingenFederal Republic of Germany

Personalised recommendations