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Current Microbiology

, Volume 29, Issue 1, pp 7–12 | Cite as

Isolation and characterization ofMethanobrevibacter oralis sp. nov.

  • Annamaria Ferrari
  • Tullio Brusa
  • Anna Rutili
  • Enrica Canzi
  • Bruno Biavati
Article

Abstract

A new coccobacillary, nonmotile, Gram-positive, methane-producing organism was isolated from human subgingival plaque. Both hydrogen and carbon dioxide were required for growth. No methane was produced from acetate, formate, or methanol. The optimum pH was 6.9–7.4, and the optimum temperature was 36–38°C. Fecal extract was required for growth, and a volatile fatty acid mixture was highly stimulatory. The DNA G+C content was 28 mol%. On the basis of these characteristics, DNA-DNA hybridization studies, and electrophoretic analysis of cellular proteins, the isolate was considered a new species and namedMethanobrevibacter oralis.

Keywords

Hydrogen Acetate Methanol Methane Dioxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Annamaria Ferrari
    • 1
  • Tullio Brusa
    • 1
  • Anna Rutili
    • 1
  • Enrica Canzi
    • 1
  • Bruno Biavati
    • 2
  1. 1.Department of Food Science and Microbiology, Section of Agricultural, Food and Ecological MicrobiologyUniversity of MilanMilanItaly
  2. 2.Institute of Agricultural MicrobiologyUniversity of BolognaBolognaItaly

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