Advertisement

Current Microbiology

, Volume 29, Issue 4, pp 223–227 | Cite as

Cloning vector system forNocardia spp.

  • Wensheng Yao
  • Yunliu Yang
  • Juishen Chiao
Article

Abstract

An electro-transformation system has been developed forNocardia asteroides andNocardia corallina by using aNocardia-Escherichia coli shuttle vector. The shuttle vector, named pCY104, was constructed by joining a 2.5-kb crypticN. asteroides plasmid pCY101 with theE. coli plasmid pIJ4625. The resistance genes for kanamycin, chloramphenicol, and thiostrepton on plasmid pCY104 were expressed inN. asteroides andN. corallina. The transformation method was optimized forN. asteroides, and transformation efficiency of 8×104 transformants per μg plasmid DNA was achieved routinely.

Keywords

Resistance Gene Kanamycin Chloramphenicol Transformation Efficiency Transformation Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Bonacini M, Walden JM (1990)Nocardia brasiliensis peritonitis in a patient with AIDS. Am J. Gastroenterol 85:1432–1433PubMedGoogle Scholar
  2. 2.
    Coque J-JR, Liras P, Laiz L, Martin JF (1991) A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes inNocardia lactamdurans. J Bacteriol 173:6258–6264PubMedGoogle Scholar
  3. 3.
    Dower WJ, Miller JF, Ragsdale CW (1988) High efficient transformation ofE. coli by high voltage electroporation. Nucleic Acids Res 16:6127–6145PubMedGoogle Scholar
  4. 4.
    Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempt H (1985) Genetic manipulation ofStreptomyces: a laboratory manual. Norwich, England. The John Innes FoundationGoogle Scholar
  5. 5.
    Hopwood DA, Bibb MJ, Chater KF, Kieser T (1987) Plasmid and phage vector for gene cloning and analysis inStreptomyces. Methods Enzymol 153:116–166PubMedGoogle Scholar
  6. 6.
    Ishihashi Y, Watanabe R, Hommura S, Koyama A, Ishikawa T, Mikami Y (1990) EndogenousNocardia asteroides endophtalmitis. Br J Ophthalmol 74:433–436PubMedGoogle Scholar
  7. 7.
    Itagaki E, Wakabayashi T, Hatta T (1990) Purification and characterization of 3-ketosteroid-delta super(1)-dehydrogenase fromNocardia corallina. Biochim Biophys Acta 1039: 60–67Google Scholar
  8. 8.
    Kawakawi K (1990) Characterization of production of 1,2-epoxyoctane from 1-octene by nongrowing cells ofNocardia corallina B-176 in aqueous-organic media. Enzyme Eng 10:707–711Google Scholar
  9. 9.
    Kieser T (1984) Factors affecting the isolation of cccDNA fromStreptomyces lividans andEscherichia coli. Plasmid 12:19–36PubMedGoogle Scholar
  10. 10.
    Lechevalier H (1989) Nocardioform Actinomycetes in: Williams ST, Sharpe ME, Holt JG (eds), Bergey's manual of systematic bacteriology vol 4. Baltimore, Williams & Wilkins, pp 2348–2404Google Scholar
  11. 11.
    Liechty MC, Bradley SG, Kasweck KL (1987) Genetic recombination by protoplast fusion inNocardia asteroides. J Ind Microbiol 2:71–77Google Scholar
  12. 12.
    McNeil MM, Brown JM, Magruder CH, Shearlock KT, Saul RA, Allred DP, Ajello L (1992) DisseminatedNocardia transvalensis infection: an unusual opportunistic pathogen in severely immunocompromised patients. J Infect Dis 165:175–178PubMedGoogle Scholar
  13. 13.
    Meletzus D, Eichenlaub R (1991) Transformation of phytopathogenic bacteriumClavibacter michiganense subsp.michiganense by electroporation and development of a cloning vector. J Bacteriol 173:184–190PubMedGoogle Scholar
  14. 14.
    Miller JF, Dower WJ, Tompkins LS (1988) High voltage electroporation of bacteria: genetic transformation ofCampylobacter jejuni with plasmid DNA. Proc Natl Acad Sci USA 85:856–860PubMedGoogle Scholar
  15. 15.
    Projan SJ, Carleton S, Novick RP (1983) Determination of plasmid copy number by fluorescence densitometry. Plasmid 9:182–190PubMedGoogle Scholar
  16. 16.
    sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory PressGoogle Scholar
  17. 17.
    Schupp T, Divers M (1986) Protoplast preparation and regeneration inNocardia mediterranei. FEMS Microbiol Lett 36:159–162Google Scholar
  18. 18.
    Tarnok, I (1976) Metabolism in nocardiae and related bacteria. In: Goodfellow M, Brownell GH, Serrano JA (eds) The biology of the nocardiae. New York: Academic Press, pp 451–500Google Scholar
  19. 19.
    Thompson CT, Ward JM, Hopwood DA (1982) Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol 151:668–677PubMedGoogle Scholar
  20. 20.
    Weijers CAGM, deBont JAM (1991) Enantioselective degradation of 1,2-epoxyalkanes byNocardia H8. Enzyme Microb Technol 13:306–308Google Scholar
  21. 21.
    Williamson JM, Hock D, Inamine ES (1991) HMG-COA reductase inhibitors produced byNocardia sp. (MA6455). US Patent 4, 997, 755Google Scholar
  22. 22.
    Yao WS, Yang YL, Chiao JS (1992) IS204: an insertion sequence fromN. asteroides (mexicana) IFO3927. To be publishedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1994

Authors and Affiliations

  • Wensheng Yao
    • 1
  • Yunliu Yang
    • 1
  • Juishen Chiao
    • 1
  1. 1.Shanghai Institute of Plant PhysiologyChinese AcademyShanghaiP.R. China

Personalised recommendations