Current Microbiology

, Volume 29, Issue 4, pp 223–227 | Cite as

Cloning vector system forNocardia spp.

  • Wensheng Yao
  • Yunliu Yang
  • Juishen Chiao


An electro-transformation system has been developed forNocardia asteroides andNocardia corallina by using aNocardia-Escherichia coli shuttle vector. The shuttle vector, named pCY104, was constructed by joining a 2.5-kb crypticN. asteroides plasmid pCY101 with theE. coli plasmid pIJ4625. The resistance genes for kanamycin, chloramphenicol, and thiostrepton on plasmid pCY104 were expressed inN. asteroides andN. corallina. The transformation method was optimized forN. asteroides, and transformation efficiency of 8×104 transformants per μg plasmid DNA was achieved routinely.


Resistance Gene Kanamycin Chloramphenicol Transformation Efficiency Transformation Method 
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Copyright information

© Springer-Verlag New York Inc. 1994

Authors and Affiliations

  • Wensheng Yao
    • 1
  • Yunliu Yang
    • 1
  • Juishen Chiao
    • 1
  1. 1.Shanghai Institute of Plant PhysiologyChinese AcademyShanghaiP.R. China

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