Journal of Industrial Microbiology

, Volume 17, Issue 1, pp 47–52 | Cite as

A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis inEscherichia coli

  • G Gosset
  • J Yong-Xiao
  • A Berry


Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains ofEscherichia coli. In a strain having a wild-type PEP: glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase (pykA andpykF) resulted in a 3.4-fold increase in carbon flow to aromatic biosynthesis. In a strain already having increased carbon flow to aromatics by virtue of overexpression of thetktA gene (encoding transketolase), thepykA and/orpykF mutations had no effect. A PTS glucose+ mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS+ control strain. In the PTS glucose+ host background, overexpression oftktA caused a further 3.7-fold increase in carbon flow, while inactivation ofpykA andpykF caused a 5.8-fold increase. When all of the variables tested (PTS glucose+,pykA, pykF, and overexpressedtktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved.


amino acids aromatics E. coli DAHP PEP 


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Copyright information

© Society for Industrial Microbiology 1996

Authors and Affiliations

  • G Gosset
    • 1
  • J Yong-Xiao
    • 2
  • A Berry
    • 2
  1. 1.Instituto de BiotecnologiaUniversidad Nacional Autónoma de MéxicoCuernavacaMéxico
  2. 2.Genencor InternationalPalo AltoUSA

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