Journal of Industrial Microbiology

, Volume 15, Issue 6, pp 498–502 | Cite as

Fermentation of raw glycerol to 1,3-propanediol by new strains ofClostridium butyricum

  • E Petitdemange
  • C Dürr
  • S Abbad Andaloussi
  • G Raval


Industrial glycerol obtained through the transesterification process using rapeseed oil did not support growth of several strains ofClostridium butyricum obtained from bacterial culture collections. Ten new strains ofC. butyricum were obtained from mud samples from a river, a stagnant pond, and a dry canal. These new isolates fermented the commercial glycerol and produced 1,3-propanediol as a major fermentation product with concomitant production of acetic and butyric acids. Four of the ten isolates were able to grow on industrial glycerol obtained from rapeseed oil. One strain,C. butyricum E5, was very resistant to high levels of glycerol and 1,3-propanediol. Using fed-batch fermentation, 109 g L−1 of industrial glycerol were converted into 58 g of 1,3-propanediol, 2.2 g of acetate and 6.1 g of butyrate per liter.


Clostridium butyricum glycerol 1,3-propanediol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Biebl H. 1991. Glycerol fermentation to 1,3-propanediol byClostridium butyricum. Measurement of product inhibition by use of pH-auxostat. appl Microbiol Biotechnol 35:701–705.Google Scholar
  2. 2.
    Biebl H, S Marten, H Hippe and W-D Deckwer. 1992. Glycerol conversion to 1,3-propanediol by newly isolated clostridia. Appl Microbiol Biotechnol 36: 592–597.Google Scholar
  3. 3.
    Biebl H and N Pfenning. 1981. Isolation of members of the family Rhodospirillaceae. In: The Prokaryotes (Starr MP, H Stolp, HG Trüper, A Balows and HG Schlegel, eds), pp 267–273. Springer, Berlin, Heidelberg, New York.Google Scholar
  4. 4.
    Dabrock B, H Bahl and G Gottschalk. 1992. Parameters affecting solvent production byClostridium pasteurianum. Appl Environ Microbiol 58: 1233–1239.Google Scholar
  5. 5.
    Deckwer W-D, B Günzel, H Biebl, RJ Müller and FJ Carduck. 1992. Glycerol conversion to 1,3-propanediol, a versatile component for biodegradable plastics. In: Biomass for Energy and Industry (Hall DO, G Grassi and H Scheer, eds), pp 326–331. Elsevier Applied Science. London and New York.Google Scholar
  6. 6.
    Forage RG and MA Foster. 1982. Glycerol fermentation inKlebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and diol dehydratases. J Bacteriol 149: 413–419.PubMedGoogle Scholar
  7. 7.
    Forsberg CW. 1987. Production of 1,3-propanediol from glycerol byClostridium acetobutylicum and otherClostridium species. Appl Environ Microbiol 53: 639–643.Google Scholar
  8. 8.
    Günzel B, S Yonsel and W-D Deckwer. 1991. Fermentative production of 1,3-propanediol from glycerol byClostridium butyricum up to scale of 2 m3. Appl Microbiol Biotechnol 36: 289–294.Google Scholar
  9. 9.
    Heyndrickx M, P de Vos, M Vancanneyt and J de Ley. 1991. The fermentation of glycerol byClostridium butyricum LMG 1212 t2 and 1213 t1 andC. pasteurianum LMG 3285. Appl Microbiol Biotechnol 34: 637–642.Google Scholar
  10. 10.
    Holdeman LV and WEC Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute, Blacksburg, VA.Google Scholar
  11. 11.
    Homann, T, C Tag, H Biebl, W-D Deckwer and B Schink. 1990. Fermentation of glycerol to 1,3-propanediol byKlebsiella andCitrobacter strains. Appl Microbiol Biotechnol 33: 121–126.Google Scholar
  12. 12.
    Schütz H and F Radler. 1984. Anaerobic reduction of glycerol to propanediol-1,3 byLactobacillus brevis andLactobacillus buchner. Syst Appl Microbiol 5: 169–178.Google Scholar
  13. 13.
    Streekstra H, MJ Teixeira de Mattos, OM Neijssel and DW Tempest. 1987. Overflow metabolism during anaerobic growth ofKlebsiella aerogenes NCTC 418 on glycerol and dihydroxyacetone in Chemostat culture. Arch Microbiol 147: 268–275.Google Scholar
  14. 14.
    Zeng AP, A Ross, H Biebl, C Tag, B Günzel and W-D Deckwer 1994. Multiple product inhibition and growth modeling ofClostridium butyricum andKlebsiella pneumoniae in glycerol fermentation. Biotechnol Bioeng 44: 902–911.Google Scholar

Copyright information

© Society for Industrial Microbiology 1995

Authors and Affiliations

  • E Petitdemange
    • 1
  • C Dürr
    • 1
  • S Abbad Andaloussi
    • 1
  • G Raval
    • 1
  1. 1.Laboratoire de Chimie BiologiqueFaculté des SciencesVandoeuvre-les-Nancy, CédexFrance

Personalised recommendations