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Journal of Industrial Microbiology

, Volume 16, Issue 3, pp 145–154 | Cite as

Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter

  • R S Donovan
  • C W Robinson
  • B R Glick
Article

Abstract

This review examines factors which influence the expression of foreign proteins inEscherichia coli under the transcriptional control of thelac andtac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-β-d-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.

Keywords

lac promoter tac promoter recombinant DNA protein overexpression fermentation strategies IPTG lactose 

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Copyright information

© Society for Industrial Microbiology 1996

Authors and Affiliations

  • R S Donovan
    • 1
    • 2
  • C W Robinson
    • 1
    • 2
  • B R Glick
    • 2
    • 1
  1. 1.Department of Chemical EngineeringUniversity of WaterlooWaterlooCanada
  2. 2.Department of BiologyUniversity of WaterlooWaterlooCanada

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