Journal of Industrial Microbiology

, Volume 15, Issue 5, pp 449–452 | Cite as

Bioassay strain development for the analysis of bacitracin in bacitracin/chlortetracycline combinations

  • J J Mathers
  • A D Desai
Article
Received:
Accepted:

Abstract

A variant strain was developed fromMicrococcus luteus ATCC 10240 for the purpose of bioassay analysis of bacitracin in the presence of chlortetracycline (CTC). Strain EN5 resulted from four sequential mutation steps, using quantitative resistance to CTC and retained bacitracin sensitivity as a selective criterion. Strain EN5 was tested for bioassay response, stability, and identity. The strain measured bacitracin activity with no interference from 40 μg ml−1 added CTC.

Keywords

antibiotics bacitracin chlortetracycline bioassay interference strain development 
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Copyright information

© Society for Industrial Microbiology 1995

Authors and Affiliations

  • J J Mathers
    • 1
  • A D Desai
    • 1
  1. 1.Animal Health DivisionALPHARMA IncChicago HeightsUSA

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