Journal of Industrial Microbiology

, Volume 14, Issue 3–4, pp 203–207 | Cite as

Remediation of uranium contaminated soils with bicarbonate extraction and microbial U(VI) reduction

  • Elizabeth J. P. Phillips
  • Edward R. Landa
  • Derek R. Lovley
Article

Summary

A process for concentrating uranium from contaminated soils in which the uranium is first extracted with bicarbonate and then the extracted uranium is precipitated with U(VI)-reducing microorganisms was evaluated for a variety of uranuum-contaminated soils. Bicarbonate (100 mM) extracted 20–94% of the uranium that was extracted with nitric acid. The U(VI)-reducing microorganism,Desulfovibrio desulfuricans reduced the U(VI) to U(IV) in the bicarbonate extracts. In some instances unidentified dissolved extracted components, presumably organics, gave the extract a yellow color and inhibited U(VI) reduction and/or the precipitation of U(IV). Removal of the dissolved yellow material with the addition of hydrogen peroxide alleviated this inhibition. These results demonstrate that bicarbonate extraction of uranium from soil followed by microbial U(VI) reduction might be an effective mechanism for concentrating uranium from some contaminated soils.

Key words

Bioremediation Uranium Mill tailings Desulfovibrio 

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References

  1. 1.
    Allison, L.E. and C.D. Moody, 1965. In: Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties (Black, C.A., ed.), pp. 1379–1396, American Society of Agronomy. Madison, WI.Google Scholar
  2. 2.
    Cox, C.H. and W.J. Roushey. 1979. Recovery of uranium by in situ solution mining. Min. Indust. Bull. 22: 1–12.Google Scholar
  3. 3.
    Ebinger, M.H., E.H. Essington, E.S. Gladney, B.D. Newman and C. Reynolds. 1993. Redistribution of depleted uranium (DU) in soils and water at two US Army proving grounds. Ann. Meet. Health Phys. Soc. Absts. 64: S60.Google Scholar
  4. 4.
    Gorby, Y.A. and D.R. Lovley. 1992. Enzymatic uranium precipitation. Environ. Sci. Technol. 26: 205–207.Google Scholar
  5. 5.
    Landa, E.R. 1982. Leaching of radionuclides from uranium ore and mill tailings. Uranium 1: 53–64.Google Scholar
  6. 6.
    Landa, E.R. 1984. Geochemical and radiological characterization of soils from former radium processing sites. Health Phys. 46: 385–394.PubMedGoogle Scholar
  7. 7.
    Landa, E.R. 1987. Radium-226 contents and Rn emanation coefficients of particle-size fractions of alkaline, acid and mixed U mill tailings. Health Phys. 52: 303–310.PubMedGoogle Scholar
  8. 8.
    Lovley, D.R. and E.J.P. Phillips. 1992. Bioremediation of uranium contamination with enzymatic uranium reduction. Eviron. Sci. Technol. 26: 2228–2234.Google Scholar
  9. 9.
    Lovley, D.R. and E.J.P. Phillips. 1992. Reduction of uranium byDesulfovibrio desulfuricans. Appl. Environ. Microbiol. 58: 850–856.PubMedGoogle Scholar
  10. 10.
    Lovley, D.R., E.J.P. Phillips, Y.A. Gorby and E.R. Landa. 1991. Microbial reduction of uranium. Nature 350: 413–416.Google Scholar
  11. 11.
    Lovley, D.R., E.E. Roden, E.J.P. Phillips and J.C. Woodward. 1993. Enzymatic iron and uranium reduction by sulfate-reducing bacteria. Marine Geol. 113: 41–53.Google Scholar
  12. 12.
    Lovley, D.R., P.K. Widman, J.C. Woodward and J.P. Phillips. 1993. Reduction of uranium by cytochromec 3 ofDesulfovibrio vulgaris. Appl. Environ. Microbiol. 59: 3572–3576.PubMedGoogle Scholar
  13. 13.
    Osiensky, J.L. and R.E. Williams. 1990. Factors affecting efficient aquifer restoration at in situ uranium mine sites. Ground Wat. Mont Rev. 10: 107–112.Google Scholar
  14. 14.
    Sheppard, S.C. and W.G. Evenden. 1992. Bioavailability indices for uranium: effect of concentration in eleven soils. Arch. Environ. Contam. Toxicol. 23: 117–124.Google Scholar

Copyright information

© Society for Industrial Microbiology 1995

Authors and Affiliations

  • Elizabeth J. P. Phillips
    • 1
  • Edward R. Landa
    • 1
  • Derek R. Lovley
    • 1
  1. 1.430 National CenterUS Geological SurveyRestonUSA

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