Methionine induction of ACV synthetase inCephalosporium acremonium
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Summary
Methionine markedly stimulates the biosynthesis of penicillin N and cephalosporin C inCephalosporium acremonium. Examination of intra- and extracellular ACV tripeptide in non-producing mutant N-2 showed that growth in the presence of methionine increased ACV accumulation. Direct measurement of ACV synthetase activity in a cell-free system indicated that the methionine effect was mainly due to induction of this first enzyme of the β-lactam biosynthetic pathway, resulting in a corresponding increase in β-lactam production in both a low-producing strain and a high-producing mutant.
Key words
Methionine effect ACV accumulation β-Lactam productionPreview
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References
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© Society for Industrial Microbiology 1987