Journal of Industrial Microbiology

, Volume 1, Issue 1, pp 23–29 | Cite as

Degradation of highly chlorinated PCBs byPseudomonas strain LB400

  • Lawrence H. Bopp
Original Papers


Congeners of polychlorinated biphenyl (PCB) differ in the number and position of chlorine substituents. Although PCBs are degraded, those congoners with five or more chlorines have been considered resistant to bacterial degradation. Metabolism byPseudomonas strain LB400 of PCBs representing a broad spectrum of chlorination patterns and having from two to six chlorines was investigated. Degradation of pure PCB congeners and synthetic congener mixes was measured in resting cell assays with biphenyl- or Luria broth-grown cells. In addition, the appearance of metabolites was followed using HPLC purification, and GC and GC-MS characterization. 2,4,5,2′,4′,5′-[14C]hexachlorobiphenyl was also used to follow the accumulation of14C-labeled metabolites. Evidence indicates that LB400 aerobically metabolizes representatives of all major structural classes of PCB's including several congeners which lack adjacent unchlorinated carbon atoms. The mechanisms by which many of these congeners are degraded are not fully understood, but it is apparent that aerobic bacteria can degrade a broader spectrum of PCB congeners than previously believed and that this broad spectrum of degradative competence can exist in a single strain.

Key words

PCB Degradation Metabolism Pseudomonas 


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  1. 1.
    Bedard, D.B., M.J. Brennan and R.D. Unterman. 1984. Bacterial degradation of PCBs: evidence of distinct pathways inCorynebacterium sp. MB1 andAlcaligenes eutrophus H850. In: Proceedings: 1983 PCB Seminar. (Addis, G. and R.Y. Komai, eds.), pp. 4–101—4–118. Electric Power Research Institute. Palo Alto, CA.Google Scholar
  2. 2.
    Bopp, L.H., A.M. Chakrabarty and H.L. Ehrlich. 1983. Chromatate resistance plasmid inPseudomonas fluorescens. J. Bacteriol. 155: 1105–1109.PubMedGoogle Scholar
  3. 3.
    Capel, P.D., R.A. Rapaport, S.J. Eisenreich and B.B. Looney. 1985. PCBQ: computerized quantification of total PCB and congeners in environmental samples. Chemosphere 14: 439–450.Google Scholar
  4. 4.
    Furukawa, K. 1982. Microbial degradation of polychlorinated biphenyls (PCBs). In: Biodegradation and Detoxification of environmental Pollutants. (Chakrabarty, A.M., ed.), pp. 33–57. CRC Press, Boca Raton, FL.Google Scholar
  5. 5.
    Furukawa, K., F. Matsmura and Tonomura. 1978.Alcaligenes andAcinetobacter strains capable of degrading polychlorinated biphenyls. Agric. Biol. Chem. 42: 543–548.Google Scholar
  6. 6.
    Furukawa, K., N. Tomizuka and A. Kamibayashi. 1979. Effects of chlorine substitution pattern on the bacterial metabolism of various polychlorinated biphenyls. Appl. Environ. Microbiol. 38: 301–310.PubMedGoogle Scholar
  7. 7.
    Johnston, H.W., G.G. Briggs and M. Alexander. 1972. Metabolism of 3-chlorobenzoic acid by a Pseudomonad. Soid Biol. Biochem. 4: 187–190.Google Scholar
  8. 8.
    Kato, S., J.D. McKinney and H.B. Matthews. 1980. Metabolism of symmetrical hexachlorobiphenyl isomers in the rat. Toxiol. Appl. Pharmacol. 53: 389–398.Google Scholar
  9. 9.
    Matthews, H.B. and S. Kato. 1979. The metabolism and disposition of halogenated aromatics. Ann. N.Y. Acad. Sci. 320: 131–137.PubMedGoogle Scholar
  10. 10.
    Matthews, H.B. and D.B., Tuey. 1980. The effect of chlorine position on the distribution and excretion of four hexachlorobipenyl isomers. Toxicol. Appl. Pharmacol. 53: 377–388.PubMedGoogle Scholar
  11. 11.
    Preston, B.D., J.A. Miller and E.C. Miller. 1983. Non-arene oxide aromatic ring hydroxylation of 2,2′,5,5′-tetrachlorobiphenyl as a major metabolic pathway catalyzed by phenobarbitol-induced rat liver microsomes. J. Biolo. Chem. 258: 8304–8311.Google Scholar
  12. 12.
    Sayler, G.S., M. Shon and R.R. Colwell. 1977. Growth of an eustuarinePseudomonas sp. on polychlorinated biphenyl. Microbiol. Ecol. 3: 241–255.Google Scholar
  13. 13.
    Sundstrom, G., O. Hutzinger and S. Safe. 1976. The metabolism of 2,2′,4,4′,5,5′-hexachlorobiphenyl by rabbits, rats and mice. Chemosphere 4: 249–253.Google Scholar
  14. 14.
    Sundstrom, G., O. Hutzinger and S. Safe. 1976b. The metabolism of chlorobiphenyls — a review. Chemosphere 5: 267–298.Google Scholar
  15. 15.
    Sylvestre, M., R. Masse, F. Messier, J. Fauteux, J.-G. Bisaillon and R. Beaudet. 1982. Bacterial nitration of 4-chlorobiphenyl. Appl. Environ. Microbiol. 44: 871–877.Google Scholar

Copyright information

© Society for Industrial Microbiology 1986

Authors and Affiliations

  • Lawrence H. Bopp
    • 1
  1. 1.Corporate Research and DevelopmentGeneral Electric CompanySchenectadyU.S.A.

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