Journal of Industrial Microbiology

, Volume 2, Issue 1, pp 15–23

A proton-deuterium exchange study of three types ofDesulfovibrio hydrogenases

  • Guy D. Fauque
  • Yves M. Berlier
  • Melvin H. Czechowski
  • Bernard Dimon
  • Paul A. Lespinat
  • Jean LeGall
Original Papers

DOI: 10.1007/BF01569401

Cite this article as:
Fauque, G.D., Berlier, Y.M., Czechowski, M.H. et al. Journal of Industrial Microbiology (1987) 2: 15. doi:10.1007/BF01569401

Summary

Hydrogenases are among the main enzymes involved in bacterial anaerobic corrosion of metals. The study of their mode of action is important for a full comprehension of this phenomenon. The three types ofDesulfovibrio hydrogenases [(Fe), (NiFe), (NiFeSe)] present different patterns in the pH dependence of their activity. The periplasmic enzyme fromDesulfovibrio salexigens and the cytoplasmic enzyme fromDesulfovibrio baculatus both have pH optima at 7.5 for H2 uptake and 4.0 for H2 evolution and H+−D2 exchange reaction (measured by membrane-inlet mass-spectrometry). The H2 to HD ratio at pH above 5.0 is higher than 1.0. The periplasmic hydrogenase fromD. gigas presents the same pH optimum (8.0) for the H+−D2 exchange as for H2 consumption. In contrast, the enzyme fromD. vulgaris has the highest activity in H2 production and in the exchange at pH 5.0. Both hydrogenases have a H2-to-HD ratio below 1.0.

Key words

Hydrogenase pH Proton-deuterium exchange Desulfovibrio Anaerobic corrosion Membrane-inlet mass-spectrometry 

Copyright information

© Society for Industrial Microbiology 1987

Authors and Affiliations

  • Guy D. Fauque
    • 2
    • 1
  • Yves M. Berlier
    • 2
  • Melvin H. Czechowski
    • 1
  • Bernard Dimon
    • 2
  • Paul A. Lespinat
    • 2
  • Jean LeGall
    • 2
    • 1
  1. 1.Department of Biochemistry, School of Chemical SciencesUniversity of Georgia, Boyd Graduate Studies Research CenterAthensU.S.A.
  2. 2.A.R.B.S.-Equipe Commune d'Enzymologie CNRS-CEAC.E.N. CadaracheSaint-Paul-lez-Durance CedexFrance

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