Journal of Industrial Microbiology

, Volume 1, Issue 6, pp 349–353 | Cite as

Production of propionic acid by mixed cultures ofPropionibacterium shermanii andLactobacillus casei in autoclave-sterilized whey

  • Elizabeth A. Bodie
  • Nelson Goodman
  • Robert D. Schwartz
Original Papers

Summary

Pure cultures ofPropionibacterium freudenreichii ss.shermanii did not grow in autoclave-sterilized cheese whey (121°C, 15 psi, 20 min) at whey concentrations greater than 2% (w/v) spray-dried sweet dairy whey. Propionic acid was produced from autoclave-sterilized whey by growingP. shermanii in mixed culture withLactobacillus casei. In medium containing 5–12% autoclaved whey solids and 1% yeast extract, the mixed culture produced 1.3–3.0% propionic acid, 0.5–1.0% acetic acid, and 0.05–0.80% lactic acid. All the lactose was consumed. Using pH-controlled fermentors (pH=7.0), mixed cultures produced at least 30% more propionic acid than cultures in which pH was not controlled.

Key words

Propionic acid Fermentation 

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References

  1. 1.
    Al-ani, M.R., H.E. Clark and J.M. Howe. 1972. Evaluation of whey as a protein supplement for wheat flour. Nutr. Rep. Int. 5:111–118.Google Scholar
  2. 2.
    Anderson, T.M., E.A. Bodie, N. Goodman and R.D. Schwartz. 1986. Inhibitory effect of autoclaving whey-based medium on propionic acid production byPropionibacterium shermanii. Appl. Environ. Microbiol. 51:427–428.Google Scholar
  3. 3.
    Bullerman, L.B. and E.E. Berry. 1966. Use of cheese whey for vitamin B12 production. Appl. Microbiol. 14:353–355.PubMedGoogle Scholar
  4. 4.
    Gordon, W.G. and E.B. Kalan. 1974 Proteins of milk. In: Fundamentals of Dairy Chemistry, 2nd Edn. (Webb, B.H., A.H. Johnson and J.A. Alford, eds.), pp. 102–103, The AVI Publishing Company, Westport, CT.Google Scholar
  5. 5.
    Hettinga, D.H. and G.W. Reinbold. 1972. The propionic acid bacteria — a review. II. Metabolism. J. Milk Food Technol. 35:352–372.Google Scholar
  6. 6.
    Huhtanen, C.N., F.W. Parrish and K.B. Hicks. 1980. Inhibition of bacteria by lactulose preparations. Appl. Environ. Microbiol. 40:171–173.Google Scholar
  7. 7.
    Lee, I.H., A.G. Fredrickson and H.M. Tsuchiya. 1974. Diauxic growth ofPropionibacterium shermanii. Appl. Microbiol. 28:831–835.PubMedGoogle Scholar
  8. 8.
    Lee, I.H., A.G. Frederickson and H.M. Tsuchiya. 1976. Dynamics of mixed cultures ofLactobacillus plantarum andPropionibacterium shermanii. Biotechnol. Bioeng. 18:513–526.PubMedGoogle Scholar
  9. 9.
    Leviton, A., and R.E. Hargrove. 1952. Microbiological synthesis of vitamin B12 by propionic acid bacteria. Ind. Eng. Chem. 44:2651–2655.Google Scholar
  10. 10.
    Liu, J.P. and N.J. Moon. 1982. Commensalistic interaction betweenLactobacillus acidophilus andPropionibacterium shermanii. Appl. Environ. Microbiol. 44:715–722.Google Scholar
  11. 11.
    Michels, P.A., J.P. Michels, J. Boonstra and W.N. Konings. 1979 Generation of an electrochemical proton gradient in bacteria by the excretion of metabolic end products. FEMS Microbiol. Lett. 5:357–364.Google Scholar
  12. 12.
    Moon, N.J., L.O. Ely and Z.M. Sodweeks. 1980. Aerobic deterioration of wheat, lucerne and maize silages prepared withLactobacillus acidophilus and aCandida spp. J. Appl. Bacteriol. 49:75–87.Google Scholar
  13. 13.
    Otto, R., J. Hugenholtz, W.N. Konings and H. Veldkamp. 1980. Increase of molar growth yield ofStreptococcus cremoris for lactose as a consequence of lactate consumption byPseudomonas stutzeri in mixed culture. FEMS Microbiol. Lett. 9:85–88.Google Scholar
  14. 14.
    Parker J.A. and N.J. Moon. Interactions ofLactobacillus andPropionibacterium in mixed culture. J. Food Protect. 45:326–330.Google Scholar
  15. 15.
    Sandine, W.E. 1977. New techniques in handling lactic cultures to enhance their performance. J. Dairy Sci. 60:822–828.Google Scholar
  16. 16.
    Schwartz, R.D. and E.A. Bodie. 1985. Production of high-viscosity whey broths by a lactose-utilizingXanthomonas campestris strain. Appl. Environ. Microbiol. 50:1483–1485.Google Scholar
  17. 17.
    Sherman, J.M. 1932. Propionic acid fermentation by the use of mixed strains of propionic bacteria. U. S. Patent 1,865,146.Google Scholar
  18. 18.
    Sherman, J.M. and R.H. Shaw. 1923. The propionic acid fermentation of lactose. J. Biol. Chem. 56:695–709.Google Scholar
  19. 19.
    Singh, J. and B. Rauganathan. 1978. A comparison of the activity ofLactobacillus bulgaricus and one of its mutants in different types of milk. J. Dairy Res. 45:123–125.Google Scholar
  20. 20.
    Thayanithy, K., G. Harding and D.A. Wase. 1982. Rearrangement of lactose on sterlization. Bio. Technol. Lett. 4: 423–424.Google Scholar
  21. 21.
    Vananuuat, P. and J.E. Kinsella. 1975. Production of yeast protein from crude lactose bySaccharomyces fragilis. Batch culture studies. J. Food Sci. 40:336–341.Google Scholar
  22. 22.
    Wilson, P. 1933. Propionic Acid Fermentation. U. S. Patent 1,898,329.Google Scholar

Copyright information

© Society for Industrial Microbiology 1987

Authors and Affiliations

  • Elizabeth A. Bodie
    • 1
  • Nelson Goodman
    • 1
  • Robert D. Schwartz
    • 1
  1. 1.Stauffer Chemical CompanyRichmondUSA

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